Two separate regions in the genome of the Tobacco etch virus contain determinants of the wilting response of Tabasco pepper

被引:57
作者
Chu, MH [1 ]
LopezMoya, JJ [1 ]
LlaveCorreas, C [1 ]
Pirone, TP [1 ]
机构
[1] UNIV KENTUCKY, DEPT PLANT PATHOL, LEXINGTON, KY 40546 USA
关键词
hypersensitive response;
D O I
10.1094/MPMI.1997.10.4.472
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Infection of Tabasco pepper by the tobacco etch virus (TEV) typically causes wilting associated with root necrosis, However, a strain of TEV, designated TEV nonwilting (TEV NW), is able to infect Tabasco pepper plants but does not cause wilting, In order to locate the genetic determinants responsible for the wilting response, a full-length cDNA clone of TEV NW from which infectious transcripts can be derived was made, A number of chimeric constructs were prepared by substituting cDNA fragments between TEV HAT (which causes wilting) and TEV NW clones, This approach was used to identify two wilting determinants in TEV HAT: one encompasses the 3' one-third of the P3 coding region; the other spans the 3' end of the CI, the 6-kDa protein, and the 5' end of the VPg-NIa coding regions, Substitution of both these TEV NN fragments into TEV HAT resulted in infection but not wilting of Tabasco pepper, while the replacement of either of the fragments alone did not alter the wilting response, This indicates that both TEV NW regions contain determinants necessary but not sufficient to alter the wilting response and that both must be present in order to avoid the wilting response, There was no difference between the in vitro transcription-translation products derived from constructs containing these regions from TEV HAT and TEV NW.
引用
收藏
页码:472 / 480
页数:9
相关论文
共 34 条
[1]   THE NUCLEOTIDE-SEQUENCE OF THE CODING REGION OF TOBACCO ETCH VIRUS GENOMIC RNA - EVIDENCE FOR THE SYNTHESIS OF A SINGLE POLYPROTEIN [J].
ALLISON, R ;
JOHNSTON, RE ;
DOUGHERTY, WG .
VIROLOGY, 1986, 154 (01) :9-20
[2]   CONSTRUCTION OF IN-FRAME CHIMERIC PLANT VIRAL GENES BY SIMPLIFIED PCR STRATEGIES [J].
ATREYA, CD ;
ATREYA, PL ;
PIRONE, TP .
PLANT MOLECULAR BIOLOGY, 1992, 19 (03) :517-522
[3]   AMINO-ACID SUBSTITUTIONS IN THE COAT PROTEIN RESULT IN LOSS OF INSECT TRANSMISSIBILITY OF A PLANT-VIRUS [J].
ATREYA, PL ;
ATREYA, CD ;
PIRONE, TP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) :7887-7891
[4]   SYSTEMIC INFECTIONS FOR ASSAY OF PLANT VIRUSES [J].
BRAKKE, MK .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1970, 8 :61-&
[5]   NUCLEOTIDE-SEQUENCE OF A STRAIN OF TOBACCO ETCH VIRUS THAT DOES NOT CAUSE TABASCO PEPPER WILT [J].
CHU, MH ;
JOHNSON, M ;
THORNBURY, D ;
BLACK, L ;
PIRONE, T .
VIRUS GENES, 1995, 10 (03) :283-288
[6]   MOLECULAR ANALYSIS OF POTATO-VIRUS-X ISOLATES IN RELATION TO THE POTATO HYPERSENSITIVITY GENE NX [J].
CRUZ, SS ;
BAULCOMBE, DC .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1993, 6 (06) :707-714
[7]   STRUCTURE-FUNCTION RELATIONSHIP BETWEEN TOBACCO MOSAIC-VIRUS COAT PROTEIN AND HYPERSENSITIVITY IN NICOTIANA-SYLVESTRIS [J].
CULVER, JN ;
STUBBS, G ;
DAWSON, WO .
JOURNAL OF MOLECULAR BIOLOGY, 1994, 242 (02) :130-138
[8]   VIRUS-HOST INTERACTIONS - INDUCTION OF CHLOROTIC AND NECROTIC RESPONSES IN PLANTS BY TOBAMOVIRUSES [J].
CULVER, JN ;
LINDBECK, AGC ;
DAWSON, WO .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1991, 29 :193-217
[9]   TAGGING OF PLANT POTYVIRUS REPLICATION AND MOVEMENT BY INSERTION OF BETA-GLUCURONIDASE INTO THE VIRAL POLYPROTEIN [J].
DOLJA, VV ;
MCBRIDE, HJ ;
CARRINGTON, JC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10208-10212
[10]   POTYVIRAL PROTEINS SHARE AMINO-ACID-SEQUENCE HOMOLOGY WITH PICORNAVIRAL, COMOVIRAL, AND CAULIMOVIRAL PROTEINS [J].
DOMIER, LL ;
SHAW, JG ;
RHOADS, RE .
VIROLOGY, 1987, 158 (01) :20-27