CONSTRUCTION OF IN-FRAME CHIMERIC PLANT VIRAL GENES BY SIMPLIFIED PCR STRATEGIES

被引:6
作者
ATREYA, CD
ATREYA, PL
PIRONE, TP
机构
[1] Department of Plant Pathology, University of Kentucky, Lexington, 40546, KY
关键词
CHIMERIC HYBRID GENES; INVERSE PCR; PCR; PLANT VIRUS;
D O I
10.1007/BF00023403
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The use of polymerase chain reaction (PCR)-based mutagenesis to create chimeric genes is presently not cost-effective because of the size and number of primers as well as the number of PCRs required. We have developed two strategies based on inverse PCR that exploit limited homologies between two DNA molecules to create in-frame chimeric plant viral genes. This report also contains a compilation of information useful for determining possible restriction sites at a given common dipeptide coding motif between any genes of interest.
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页码:517 / 522
页数:6
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