H-point curve isolation method for coupled liquid chromatography and UV-visible spectrophotometry

被引：25

作者：

Blasco-Gómez, F ^{[1
]}

Bosch-Reig, F ^{[1
]}

Campíns-Falcó, P ^{[1
]}

Molins-Legua, C ^{[1
]}

Herráez-Hernández, R ^{[1
]}

机构：

[1] Univ Valencia, Fac Quim, Dept Quim Analit, E-46100 Valencia, Spain

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 11期

关键词：

D O I：

10.1021/ac990649q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The H-point curve isolation method (HPCIM) for the detection of unknown interferences in chromatography is proposed. The method allows one to estimate the UV-vis spectra of interfering species in a sample as well as to test the purity of the chromatographic peaks. Besides the detection of the unknown interferences in a sample, this method allows one to calculate the concentration of an analyte in the presence of unknown compounds. To illustrate the reliability of the proposed method, samples of diuretics and amphetamines have been analyzed by normal- and reversed-phase high-performance chromatography.

引用

页码：2559 / 2565

页数：7

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