CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

被引：786

作者：

Hashimshony, Tamar ^{[1
]}

Senderovich, Naftalie ^{[1
]}

Avital, Gal ^{[1
]}

Klochendler, Agnes ^{[2
]}

de Leeuw, Yaron ^{[1
]}

Anavy, Leon ^{[1
]}

Gennert, Dave ^{[3
,4
,5
]}

Li, Shuqiang ^{[6
]}

Livak, Kenneth J. ^{[6
]}

Rozenblatt-Rosen, Orit ^{[3
,4
,5
]}

Dor, Yuval ^{[2
]}

Regev, Aviv ^{[3
,4
,5
]}

Yanai, Itai ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel

[2] Hebrew Univ Jerusalem, Hadassah Med Sch, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, IL-91010 Jerusalem, Israel

[3] Broad Inst Harvard & MIT, Klarman Cell Observ, Cambridge, MA 02142 USA

[4] MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[5] MIT, Howard Hughes Med Inst, Dept Biol, Cambridge, MA 02140 USA

[6] Fluidigm Corp, 7000 Shoreline Court,Suite 100, San Francisco, CA 94080 USA

来源：

GENOME BIOLOGY | 2016年 / 17卷

基金：

以色列科学基金会; 欧洲研究理事会;

关键词：

TRANSCRIPTOMICS;

D O I：

10.1186/s13059-016-0938-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with threefold higher sensitivity, lower costs, and less hands-on time. We implemented CEL-Seq2 on Fluidigm's C1 system, providing its first single-cell, on-chip barcoding method, and we detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2's increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use.

引用

页数：7

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