Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

被引：624

作者：

Gierahn, Todd M. ^{[1
,8
]}

Wadsworth, Marc H., II ^{[2
,3
,4
,8
]}

Hughes, Travis K. ^{[2
,3
,4
,8
]}

Bryson, Bryan D. ^{[4
,5
]}

Butler, Andrew ^{[6
,7
]}

Satija, Rahul ^{[6
,7
]}

Fortune, Sarah ^{[4
,5
]}

Love, J. Christopher ^{[1
,3
,4
]}

Shalek, Alex K. ^{[2
,3
,4
]}

机构：

[1] MIT, Koch Inst Integrat Canc Res, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[2] MIT, IMES, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[3] MIT, Dept Chem, Cambridge, MA 02139 USA

[4] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

[5] Ragon Inst MGH MIT & Harvard, Cambridge, MA USA

[6] Harvard Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA USA

[7] NYU, Dept Biol, Ctr Genom & Syst Biol, New York, NY 10003 USA

[8] New York Genome Ctr, New York, NY USA

来源：

NATURE METHODS | 2017年 / 14卷 / 04期

关键词：

EXPRESSION; HETEROGENEITY; RESPONSES; IMMUNE;

D O I：

10.1038/nmeth.4179

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.

引用

页码：395 / +

页数：8

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