Violacein-Producing Collimonas sp from the Sea Surface Microlayer of Costal Waters in Trondelag, Norway

被引：63

作者：

Hakvag, Sigrid ^{[1
]}

Fjaervik, Espen ^{[1
]}

Klinkenberg, Geir ^{[2
]}

Borgos, Sven Even F. ^{[1
,2
]}

Josefsen, Kjell D. ^{[2
]}

Ellingsen, Trond E. ^{[2
]}

Zotchev, Sergey B. ^{[1
]}

机构：

[1] Norwegian Univ Sci & Technol, Dept Biotechnol, N-7491 Trondheim, Norway

[2] SINTEF, SINTEF Ind Biotechnol, N-7034 Trondheim, Norway

来源：

MARINE DRUGS | 2009年 / 7卷 / 04期

关键词：

surface microlayer; Collimonas; violacein; biosynthetic genes; SP NOV; CHROMOBACTERIUM-VIOLACEUM; BIOSYNTHETIC-PATHWAY; TRONDHEIM FJORD; GENE-CLUSTER; BACTERIA; IDENTIFICATION; CYTOTOXICITY; PIGMENT;

D O I：

10.3390/md7040576

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A new strain belonging to the genus Collimonas was isolated from the sea surface microlayer off the coast of Trondelag, Norway. The bacterium, designated Collimonas CT, produced an antibacterial compound active against Micrococcus luteus. Subsequent studies using LC-MS identified this antibacterial compound as violacein, known to be produced by several marine-derived bacteria. Fragments of the violacein biosynthesis genes vioA and vioB were amplified by PCR from the Collimonas CT genome and sequenced. Phylogenetic analysis of these sequences demonstrated close relatedness of the Collimonas CT violacein biosynthetic gene cluster to those in Janthinobacterium lividum and Duganella sp., suggesting relatively recent horizontal gene transfer. Considering diverse biological activities of violacein, Collimonas CT shall be further studied as a potential producer of this compound.

引用

页码：576 / 588

页数：13

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