Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

被引：40

作者：

VanBuren, V

Piao, Y

Dudekula, DB

Qian, Y

Carter, MG

Martin, PR

Stagg, CA

Bassey, UC

Aiba, K

Hamatani, T

Kargul, GJ

Luo, AG

Kelso, J

Hide, W

Ko, MSH ^{[1
]}

机构：

[1] NIA, Dev Genomics & Aging Sect, Genet Lab, NIH, Bethesda, MD 20892 USA

[2] Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Bellville, South Africa

来源：

GENOME RESEARCH | 2002年 / 12卷 / 12期

关键词：

D O I：

10.1101/gr.633802

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of similar to120,000 cDNA clones, which were initially re-arrayed into a set of similar to11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

引用

页码：1999 / 2003

页数：5

共 10 条

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