Electrostatic origin of the genome packing in viruses

被引:209
作者
Belyi, Vladimir A. [1 ]
Muthukumar, M. [1 ]
机构
[1] Univ Massachusetts, Dept Polymer Sci & Engn, Amherst, MA 01003 USA
关键词
polymer assembly; capsid structure;
D O I
10.1073/pnas.0608311103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Many ssRNA/ssDNA viruses bind their genome by highly basic semiflexible pepticle arms of capsid proteins. Here, we show that nonspecific electrostatic interactions control both the length of the genome and genome conformations. Analysis of available experimental data shows that the genome length is linear in the net charge on the capsid pepticle arms, irrespective of the actual amino acid sequence, with a proportionality coefficient of 1.61 +/- 0.03. This ratio is conserved across all ssRNA/ssDNA viruses with highly basic pepticle arms, and is different from the one-to-one charge balance expected of specific binding. Genomic nucleoticles are predicted to occupy a radially symmetric spherical shell detached from the viral capsid, in agreement with experimental data.
引用
收藏
页码:17174 / 17178
页数:5
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