Localization of acyl ghrelin- and des-acyl ghrelin-immunoreactive cells in the rat stomach and their responses to intragastric pH

被引：49

作者：

Mizutani, Makoto ^{[2
]}

Atsuchi, Kaori ^{[3
]}

Asakawa, Akihiro ^{[3
]}

Matsuda, Norifumi

Fujimura, Masaki ^{[4
]}

Inui, Akio ^{[3
]}

Kato, Ikuo ^{[5
]}

Fujimiya, Mineko ^{[1
]}

机构：

[1] Sapporo Med Univ, Dept Anat, Chuo Ku, Sapporo, Hokkaido 0608556, Japan

[2] Shiga Univ Med Sci, Dept Anat, Shiga, Japan

[3] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Social & Behav Med, Kagoshima 890, Japan

[4] Daiichi Towakai Hosp, Osaka, Japan

[5] Hokuriku Univ, Fac Pharmaceut Sci, Dept Bioorgan Chem, Kanazawa, Ishikawa 92011, Japan

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2009年 / 297卷 / 05期

关键词：

GLUCOSE-TOLERANCE TEST; PLASMA GHRELIN; GASTROINTESTINAL-TRACT; ANOREXIA-NERVOSA; INSULIN; SECRETION; EXPRESSION; PEPTIDE; OBESTATIN; GLUCAGON;

D O I：

10.1152/ajpgi.00147.2009

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

100201 [内科学];

摘要：

Mizutani M, Atsuchi K, Asakawa A, Matsuda N, Fujimura M, Inui A, Kato I, Fujimiya M. Localization of acyl ghrelin- and des-acyl ghrelin-immunoreactive cells in the rat stomach and their responses to intragastric pH. Am J Physiol Gastrointest Liver Physiol 297: G974-G980, 2009. First published August 20, 2009; doi:10.1152/ajpgi.00147.2009.-Acyl ghrelin has a 28-amino acid sequence with O-n-octanoyl acid modification at the serine 3 position, whereas des-acyl ghrelin has no octanoyl acid modification. Although these peptides exert different physiological functions, no previous studies have shown the different localization of acyl ghrelin and des-acyl ghrelin in the stomach. Here we have developed an antibody specific for des-acyl ghrelin that does not crossreact with acyl ghrelin. Both acyl ghrelin- and des-acyl ghrelin-immunoreactive cells were distributed in the oxyntic and antral mucosa of the rat stomach, with higher density in the antral mucosa than oxyntic mucosa. Immunofluorescence double staining showed that acyl ghrelin- and des-acyl ghrelin-positive reactions overlapped in closed-type round cells, whereas des-acyl ghrelin-positive reaction was found in open-type cells in which acyl ghrelin was negative. Acyl ghrelin-/des-acyl ghrelin-positive closed-type cells contain obestatin; on the other hand, des-acyl ghrelin-positive open-type cells contain somatostatin. We measured the release of acyl ghrelin and des-acyl ghrelin in vascularly perfused rat stomach by ELISA, and the effects of different intragastric pH levels on the release of each peptide were examined. The release of des-acyl ghrelin from the perfused stomach was greater at pH 2 than at pH 4; however, the release of acyl ghrelin was not affected by intragastric pH. The present study demonstrated the differential localization of acyl ghrelin and des-acyl ghrelin in the rat stomach and their different responses to the intragastric pH.

引用

页码：G974 / G980

页数：7

共 29 条

[1]

Stomach regulates energy balance via acylated ghrelin and desacyl ghrelin [J].