Detection of micrometastatic cells in breast cancer by RT-PCR for the mammaglobin gene

被引：50

作者：

Bossolasco, P

Ricci, C

Farina, G

Soligo, D

Pedretti, D

Scanni, A

Deliliers, GL

机构：

[1] Osped Fatebenefratelli & Oftalm, Fdn Matarelli, I-20121 Milan, Italy

[2] Osped Maggiore, IRCCS, Ctr Trapianti Midollo, I-20122 Milan, Italy

[3] Oncol Osped Fatebenefratelli & Oftalm, I-20121 Milan, Italy

来源：

CANCER DETECTION AND PREVENTION | 2002年 / 26卷 / 01期

关键词：

breast neoplasm; mammaglobin; neoplasm circulating cells; RT-PCR;

D O I：

10.1016/S0361-090X(02)00009-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The detection of circulating cancer cells in the bone marrow (BM) and peripheral blood (PB) of patients with solid tumors may be useful for disease staging. To this aim, we evaluated the expression of the mammaglobin gene by reverse transcriptase polymerase chain reaction (RT-PCR) in 60 patients with breast cancer. Moreover, several controls were examined to test the specificity of this marker. The positive cases included 23.6% of the patients with and 9% of those without metastasis. Only 4/60 negative controls analyzed were positive by PCR. Our results show high specificity and a good correlation with disease status. (C) 2002 International Society for Preventive Oncology. Published by Elsevier Science Ltd. All fights reserved.

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页码：60 / 63

页数：4

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