In vitro production of human antigen presenting cells issued from bone marrow of patients with cancer

被引:9
作者
Coulon, V [1 ]
Ravaud, A [1 ]
Huet, S [1 ]
Gualde, N [1 ]
机构
[1] UNIV VICTOR SEGALEN,CNRS,UMR 5540,F-33076 BORDEAUX,FRANCE
来源
HEMATOLOGY AND CELL THERAPY | 1997年 / 39卷 / 05期
关键词
antigen presenting cells; human bone marrow; in vitro production;
D O I
10.1007/s00282-997-0237-7
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
In the prospect of producing autologous antigen presenting cells (APC) to actively immunize patients with cancer against their own tumor we were interested in the in vitro generation of MC and/or dendritic cells. We observed that the best yielding in CD14+ cells was obtained by adding SCF and GM-CSF into RPMI 1640 completed medium and by using Tenon bags as culture-containers. The others growth factors tested (LIF, IL3 and M-CSF) were useless in term of production of macrophages. After a month of culture we usually obtained an average of 80% of CD14, CD33, CD64, CD11a, CD11b, CD11c and HLA-DR positive cells expressing the MGG staining phenotype of MC. For DC the best association of growth factors combined GM-CSF, IL4 and SCF. Hence we could obtained at least 60% of CD1a+, CD14-, CD54+, CD58+, CD80+ and HLA DR+ dendritic cells.
引用
收藏
页码:237 / 244
页数:8
相关论文
共 58 条
[1]   CD40 EXPRESSION BY HUMAN MONOCYTES - REGULATION BY CYTOKINES AND ACTIVATION OF MONOCYTES BY THE LIGAND FOR CD40 [J].
ALDERSON, MR ;
ARMITAGE, RJ ;
TOUGH, TW ;
STROCKBINE, L ;
FANSLOW, WC ;
SPRIGGS, MK .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 178 (02) :669-674
[2]  
ANDREESEN R, 1990, CANCER RES, V50, P7450
[3]   CYTOKINES - COORDINATORS OF IMMUNE AND INFLAMMATORY RESPONSES [J].
ARAI, K ;
LEE, F ;
MIYAJIMA, A ;
MIYATAKE, S ;
ARAI, N ;
YOKOTA, T .
ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :783-836
[4]   TUMOR-NECROSIS-FACTOR RECEPTOR SUPERFAMILY MEMBERS AND THEIR LIGANDS [J].
ARMITAGE, RJ .
CURRENT OPINION IN IMMUNOLOGY, 1994, 6 (03) :407-413
[5]  
AUGER MJ, 1992, MACROPHAGE, P1
[6]   Immune therapy with macrophages: Present status and critical requirements for implementation [J].
Bartoleyns, J ;
RometLemonne, JL ;
Chokri, M ;
Lopez, M .
IMMUNOBIOLOGY, 1996, 195 (4-5) :550-562
[7]   RECENT ADVANCES IN THE STUDY OF DENDRITIC CELLS AND FOLLICULAR DENDRITIC CELLS [J].
CAUX, C ;
LIU, YJ ;
BANCHEREAU, J .
IMMUNOLOGY TODAY, 1995, 16 (01) :2-4
[8]  
CHEN BDM, 1988, BLOOD, V71, P997
[9]  
CHOKRI M, 1992, ANTICANCER RES, V12, P2257
[10]  
CLUITMANS FHM, 1993, LYMPHOKINE CYTOK RES, V12, P457