Reversed-phase high-performance liquid chromatography-electrospray mass spectrometry profiling of transgenic and non-transgenic maize for cultivar characterization

被引：12

作者：

Garcia Lopez, Ma Concepcion ^{[1
]}

Garcia-Canas, Virginia ^{[2
]}

Marina Alegre, Ma Luisa ^{[1
]}

机构：

[1] Univ Alcala, Fac Chem, Dept Analyt Chem, Madrid 28871, Spain

[2] CSIC, Dept Food Anal, Inst Ind Fermentat, E-28006 Madrid, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 43期

关键词：

HPLC-mass spectrometry; Maize profiling; Cultivar characterization; Generically modified organisms; Transgenic foods; GMOs; Bt maize; GENETICALLY-MODIFIED ORGANISMS; CAPILLARY GEL-ELECTROPHORESIS; POLYMERASE-CHAIN-REACTION; CORN PROTEIN; DNA; PERFUSION; QUANTIFICATION; MATRIX; ZEIN; PCR;

D O I：

10.1016/j.chroma.2009.08.050

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatography-electrospray mass spectrometry (RP-HPLC-ESI-MS (ion trap)) method is developed, for the first time, for profiling transgenic and non-transgenic maize with the aim of cultivar characterization. To optimize chromatographic conditions the following parameters were studied: column, gradient, and ion-pairing reagent. Moreover, the influence in the MS signal of the variation of the capillary voltage and the accumulated ions in the trap was also studied. The developed method was applied to the profiling of different protein fractions (albumin, globulin, prolamin, and glutelin) isolated from Bt transgenic and non-transgenic maize cultivars. Moreover, different maize samples, namely, maize cultivars from different geographical origins (USA, Canada, France, and Spain), transgenic maize samples with certified GMO content, and three transgenic Bt maize cultivars with their isogenic non-transgenic counterparts (Aristis Bt vs. Aristis, PR331367 vs. PR33P66, and DKC6575 vs. Tietar) were profiled by the developed method. Mass spectra obtained for certain peaks in the maize cultivars studied resulted, in some occasions, useful for cultivar characterization and differentiation. The comparison of UV and MS profiles and mass spectra corresponding to the protein fractions with those of the whole seeds enabled the assignment of some peaks. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：7222 / 7228

页数：7

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