Composite polymer particles with stimuli-responsive surface properties and specific activity of adsorbed/released trypsin

被引：19

作者：

Alam, M. Ashraful ^{[1
]}

Miah, M. A. Jalil ^{[1
]}

Ahmad, Hasan ^{[1
]}

机构：

[1] Rajshahi Univ, Dept Chem, Rajshahi 6205, Bangladesh

来源：

COLLOID AND POLYMER SCIENCE | 2007年 / 285卷 / 06期

关键词：

stimuli-responsive; composite polymer; trypsin; specific activity;

D O I：

10.1007/s00396-006-1638-9

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Micron-sized monodispersed stimuli-responsive polys' (PS)/poly(2-dimethylaminoethyl methacrylate-N-isopropyl acrylamide-ethylene glycol dimethacrylate) [P(DM-NIPAM-EGDM)] composite polymer particles were prepared by seeded copolymerisation of DM, NIPAM and EGDM with PS seed particles. Adsorption behaviour of trypsin suggested that composite particles surface has both temperature- and pH-responsive swelling-deswelling characteristics. The performance of composite polymer particles as a carrier for biomolecules in adsorption/release experiments was evaluated by measuring the specific activities of adsorbed trypsin as a function of temperature and pH.

引用

页码：715 / 720

页数：6

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