Enhanced production of inducible nitric oxide synthase by beta-glucans in mice

被引:24
作者
Hashimoto, T [1 ]
Ohno, N [1 ]
Adachi, Y [1 ]
Yadomae, T [1 ]
机构
[1] TOKYO UNIV PHARM & LIFE SCI,LAB IMMUNOPHARMACOL MICROBIAL PROD,HACHIOJI,TOKYO 19203,JAPAN
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 1997年 / 19卷 / 02期
关键词
beta-glucan; iNOS; IFN-gamma;
D O I
10.1111/j.1574-695X.1997.tb01082.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have already demonstrated that various activities including NO (nitric oxide) synthesis in vivo and in vitro significantly differ between triple helical (SPG) and single helical (alkaline-treated SPG, SPG-OH) beta-glucans. It was previously suggested that the single helical conformer of beta-glucan (SPG-OH) was dominant in cytokine production and subsequent NO synthesis in vitro. In this study, we analyzed production of inducible nitric oxide synthase (iNOS) induced by beta-glucans in vitro and in vivo. The iNOS production was enhanced in proteose peptone-induced peritoneal macrophages (PMs) cultured with SPG-OH in the presence of IFN-gamma for 24 h, and SPG-OH-induced PMs. Moreover, SPG-OH was effective for iNOS production not only in isolated macrophages but also in tissue macrophages, whereas SPG was less effective. These findings suggest that a single helical conformer is essential for iNOS production, and that NO synthesis by P-glucans is closely related to iNOS production.
引用
收藏
页码:131 / 135
页数:5
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