Estrogen Receptor α Regulates Dlx3-Mediated Osteoblast Differentiation

被引：17

作者：

Lee, Sung Ho ^{[1
]}

Oh, Kyo-Nyeo ^{[1
]}

Han, Younho ^{[1
]}

Choi, You Hee ^{[1
]}

Lee, Kwang-Youl ^{[1
]}

机构：

[1] Chonnam Natl Univ, Inst Drug Dev, Coll Pharm & Res, Gwangju 500757, South Korea

来源：

MOLECULES AND CELLS | 2016年 / 39卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Dlx3; estrogen receptor alpha; osteoblast differentiation; LIGAND-BINDING DOMAIN; CROSS-TALK; ER-ALPHA; TRANSCRIPTION FACTOR; OSTEOGENIC ACTIVITY; HOMEOBOX GENES; BONE LOSS; IN-VIVO; ACTIVATION; EXPRESSION;

D O I：

10.14348/molcells.2016.2291

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Estrogen receptor alpha (ER-alpha), which is involved in bone metabolism and breast cancer, has been shown to have transcriptional targets. Dlx3 is essential for the skeletal development and plays an important role in osteoblast differentiation. Various osteogenic stimulators and transcription factors can induce the protein expression of Dlx3. However, the regulatory function of ER-alpha in the Dlx3 mediated osteogenic process remains unknown. Therefore, we investigated the regulation of Dlx3 and found that ER-alpha is a positive regulator of Dlx3 transcription in BMP2-induced osteoblast differentiation. We also found that ER-alpha interacts with Dlx3 and increases its transcriptional activity and DNA binding affinity. Furthermore, we demonstrated that the regulation of Dlx3 activity by ER-alpha is independent of the ligand (estradiol) binding domain. These results indicate that Dlx3 is a novel target of ER-alpha, and that ER-alpha regulates the osteoblast differentiation through modulation of Dlx3 expression and/or interaction with Dlx3.

引用

页码：156 / 162

页数：7

共 46 条

[1]

Mutations in the estrogen receptor DNA-binding domain discriminate between the classical mechanism of action and cross-talk with Stat5b and activating protein 1 (AP-1) [J].