A water-soluble photocrosslinkable chitosan derivative prepared by Michael-addition reaction as a precursor for injectable hydrogel

被引：56

作者：

Gao, Xuanyue ^{[1
,2
]}

Zhou, Yingshan ^{[1
,2
]}

Ma, Guiping ^{[1
,2
]}

Shi, Suqing ^{[3
]}

Yang, Dongzhi ^{[1
,2
]}

Lu, Fengmin ^{[4
]}

Nie, Jun ^{[1
,2
]}

机构：

[1] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China

[2] Beijing Univ Chem Technol, Key Lab Beijing City Preparat & Proc Novel Polyme, Beijing 100029, Peoples R China

[3] Northwest Univ, Dept Chem, Xian 710069, Peoples R China

[4] Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 79卷 / 03期

关键词：

Chitosan; Hydrogel; Photopolymerization; Water-solubility; DRUG-DELIVERY SYSTEMS; CHEMICAL-MODIFICATION; TISSUE; SCAFFOLDS; MACROMERS; BONE;

D O I：

10.1016/j.carbpol.2009.08.033

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

With the goal of obtaining photopolymerized hydrogels for use as tissue engineering scaffolds, a water-soluble (methacryloyloxy) ethyl carboxyethyl chitosan was prepared as a photopolymerizable prepolymer through Michael-addition reaction between chitosan and ethylene glycol acrylate methacrylate. N-substitution of chitosan was verified by both H-1 NMR and C-13 NMR The degree of N-substitution, measured via 1H NMR, was easily varied from 0.10 to 0.35 by varying the molar ratio of ethylene glycol acrylate methacrylate to chitosan. Using a Vero cell line, the water-soluble photocrosslinkable chitosan derivative was found to be noncytotoxic up to a concentration of 1.0 mg/mL. The precursor was blended with D-2959 photoinitiator in solution, and UV-irradiated to create hydrogels. FTIR verified the nearly complete conversion of the double bonds in the gel. Indirect cytotoxicity assessment of the hydrogel indicated that the hydrogel was non-toxic to Vero cells. (C) 2009 Published by Elsevier Ltd.

引用

页码：507 / 512

页数：6

共 28 条

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Methacrylated glycol chitosan as a photopolymerizable biomaterial [J].