Phanquinone: a useful fluorescent pre-chromatographic derivatization reagent for liquid chromatographic analyses of amino acid dosage forms

被引：16

作者：

Gatti, R ^{[1
]}

Gioia, MG ^{[1
]}

Di Pietra, AM ^{[1
]}

机构：

[1] Univ Bologna, Dipartimento Sci Farmaceut, I-40126 Bologna, Italy

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 474卷 / 1-2期

关键词：

reversed-phase liquid chromatography; phanquinone (4,7-phenanthroline-5,6-dione); amino acids; fluorogenic pre-column derivatization;

D O I：

10.1016/S0003-2670(02)01011-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of 4,7-phenanthroline-5,6-dione (phanquinone) as a fluorogenic labeling reagent in pre-column derivatization for the LC separation of amino acids has been investigated. The compound, devoid of significant intrinsic fluorescence, reacts (60 min at 68 degreesC) with primary amino function to give fluorescent derivatives that can be separated by reversed-phase HPLC and detected at lambda(ex) = 400 run, lambda(em) = 460 nm. The experimental conditions for the derivatization and chromatographic separation are discussed. The proposed method proved to be suitable for the quality control of dosage forms (capsules, medicine in packets). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：11 / 20

页数：10

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