Mechanical measurements of a single protein molecule and human chromosomes by atomic force microscopy

被引：13

作者：

Ikai, A ^{[1
]}

Mitsui, K ^{[1
]}

Tokuoka, H ^{[1
]}

Xu, XM ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,BIODYNAM LAB,MIDORI KU,YOKOHAMA,KANAGAWA 226,JAPAN

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC MATERIALS SENSORS AND SYSTEMS | 1997年 / 4卷 / 04期

关键词：

atomic force microscopy; mechanical unfolding of proteins; Young's modulus of chromosomes;

D O I：

10.1016/S0928-4931(97)00006-4

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The force-distance mode of atomic force microscopy has been used to mechanically unfold sandwiched protein molecules between the tip and substrate. Under favorable conditions, average spring constants in the range of several tens of pN nm(-1) were obtained for the stretching event of single protein molecules. Another series of force curves was recorded to perform indentation experiments on chromosomes, by pressing down an AFM tip beyond a soft chromosomal surface. The resulting force versus depth curves were analyzed to give estimates of the Young's modulus of wet chromosomes in the range 0.5-1.1 X 10(5) N m(-2) at neutral and alkaline pH, but it was 10 times higher in acetate buffer at pH 4. (C) Elsevier Science S.A.

引用

页码：233 / 240

页数：8

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