Practical method for the multigram separation of the 5- and 6-isomers of carboxyfluorescein

被引：38

作者：

Rossi, FM

Kao, JPY

机构：

[1] UNIV MARYLAND,SCH MED,CTR MED BIOTECHNOL,BALTIMORE,MD 21201

[2] UNIV MARYLAND,SCH MED,DEPT PHYSIOL,BALTIMORE,MD 21201

来源：

BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 04期

关键词：

D O I：

10.1021/bc970078d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An efficient preparative method for separating 5- and 6-carboxyfluorescein is presented. B-Carboxyfluorescein dipivalate is isolated as its diisopropylamine salt, which can be converted to the free acid or used directly in coupling reactions. The 5-isomer is isolated from the acidified mother liquor. Isomerically pure carboxyfluoresceins are prepared by hydrolysis of the corresponding dipivalates.

引用

收藏

页码：495 / 497

页数：3

相关论文

共 11 条

[1]

AJTAI K, 1992, Biophysical Journal, V61, pA287

[2] PRODUCTION AND PROPERTIES OF SKELETAL MYOSIN SUBFRAGMENT-1 SELECTIVELY LABELED WITH FLUORESCEIN AT LYSINE-553 PROXIMAL TO THE STRONG ACTIN-BINDING SITE [J].

BERTRAND, R ;

DERANCOURT, J ;

KASSAB, R .

BIOCHEMISTRY, 1995, 34 (29) :9500-9507

[3] A BRIEF SURVEY OF METHODS FOR PREPARING PROTEIN CONJUGATES WITH DYES, HAPTENS, AND CROSS-LINKING REAGENTS [J].

BRINKLEY, M .

BIOCONJUGATE CHEMISTRY, 1992, 3 (01) :2-13

[4] CONJUGATES OF FLUORESCEIN AND SAENTA (5'-S-(2-AMINOETHYL)-N-6-(4-NITROBENZYL)-5'-THIOADENOSINE) - FLOW-CYTOMETRY PROBES FOR THE ES NUCLEOSIDE TRANSPORTER ELEMENTS OF THE PLASMA-MEMBRANE [J].

BUOLAMWINI, JK ;

CRAIK, JD ;

WILEY, JS ;

ROBINS, MJ ;

GATI, WP ;

CASS, CE ;

PATERSON, ARP .

NUCLEOSIDES & NUCLEOTIDES, 1994, 13 (1-3) :737-751

[5]

CHEN CS, 1993, J BIOL CHEM, V268, P15812

[6] HYDROPHILIC FLUORESCEIN DERIVATIVES - USEFUL REAGENTS FOR LIPOSOME IMMUNOLYTIC ASSAYS [J].

FIECHTNER, M ;

WONG, M ;

BIENIARZ, C ;

SHIPCHANDLER, MT .

ANALYTICAL BIOCHEMISTRY, 1989, 180 (01) :140-146

[7] Conjugates of Oligonucleotides and Modified Oligonucleotides: A Review of Their Synthesis and Properties [J].

Goodchild, John .

BIOCONJUGATE CHEMISTRY, 1990, 1 (03) :165-187

[8] PREPARATION OF 5-(AMINOMETHYL)FLUORESCEIN AND 6-(AMINOMETHYL)FLUORESCEIN [J].

MATTINGLY, PG .

BIOCONJUGATE CHEMISTRY, 1992, 3 (05) :430-431

[9] Fluorescein and some of its derivatives [J].

Orndorff, WR ;

Hemmer, AJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1927, 49 :1272-1280

[10]

Souto AA, 1995, ANGEW CHEM INT EDIT, V34, P2710