Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy

被引：370

作者：

Marko, Michael

Hsieh, Chyongere

Schalek, Richard

Frank, Joachim

Mannella, Carmen

机构：

[1] Wadsworth Ctr, Albany, NY 12201 USA

[2] Harvard Univ, Ctr Nanoscale Syst, Cambridge, MA 02138 USA

[3] Wadsworth Ctr, Howard Hughes Med Inst, Albany, NY 12201 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 03期

关键词：

D O I：

10.1038/nmeth1014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cryo-electron microscopy can provide high-resolution structural information about cells and organelles in the nearly native, frozen-hydrated state. Applicability, however, is limited by difficulties encountered in preparing suitably thin, vitreously frozen biological specimens. We demonstrate, by cryo-electron tomography of Escherichia coli cells, that a focused ion beam (FIB) can be used to thin whole frozen-hydrated cells in a convenient and essentially artifact-free way.

引用

收藏

页码：215 / 217

页数：3

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