Generation of oxygen gradients with arbitrary shapes in a microfluidic device

被引：91

作者：

Adler, Micha ^{[1
]}

Polinkovsky, Mark ^{[1
]}

Gutierrez, Edgar ^{[1
]}

Groisman, Alex ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Phys, La Jolla, CA 92093 USA

来源：

LAB ON A CHIP | 2010年 / 10卷 / 03期

基金：

美国国家科学基金会;

关键词：

CELL-CULTURE; POLY(DIMETHYLSILOXANE); ANGIOGENESIS; BACTERIA; HYPOXIA; SYSTEMS;

D O I：

10.1039/b920401f

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We present a system consisting of a microfluidic device made of gas-permeable polydimethylsiloxane (PDMS) with two layers of microchannels and a computer-controlled multi-channel gas mixer. Concentrations of oxygen in the liquid-filled flow channels of the device are imposed by flowing gas mixtures with desired oxygen concentrations through gas channels directly above the flow channels. Oxygen gradients with different linear, exponential, and non-monotonic shapes are generated in the same liquid-filled microchannel and reconfigured in real time. The system can be used to study directed migration of cells and the development of cell and tissue cultures under gradients of oxygen.

引用

页码：388 / 391

页数：4

共 23 条

[1]

Formation of steady-state oxygen gradients in vitro - Application to liver zonation [J].

Allen, JW ;

Bhatia, SN .

BIOTECHNOLOGY AND BIOENGINEERING, 2003, 82 (03) :253-262

[2]

Hypoxic induction of an HIF-1α-dependent bFGF autocrine loop drives angiogenesis in human endothelial cells [J].

Calvani, M ;

Rapisarda, A ;

Uranchimeg, B ;

Shoemaker, RH ;

Melillo, G .

BLOOD, 2006, 107 (07) :2705-2712

[3]

Generation of complex concentration profiles in microchannels in a logarithmically small number of steps [J].

Campbell, Kyle ;

Groisman, Alex .

LAB ON A CHIP, 2007, 7 (02) :264-272

[4]

RED LIGHT-INDUCED ACCUMULATION OF EUGLENA-GRACILIS [J].

CHECCUCCI, A ;

COLOMBETTI, G ;

DELCARRA.G ;

FERRARA, R ;

LENCI, F .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1974, 19 (03) :223-226

[5]

Generation of local concentration gradients by gas-liquid contacting [J].

de Jong, Jorrit ;

Verheijden, Pascal W. ;

Lammertink, Rob G. H. ;

Wessling, Matthias .

ANALYTICAL CHEMISTRY, 2008, 80 (09) :3190-3197

[6]

Generation of gradients having complex shapes using microfluidic networks [J].

Dertinger, SKW ;

Chiu, DT ;

Jeon, NL ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 2001, 73 (06) :1240-1246

[7]

Oxygen sensation and social feeding mediated by a C-elegans guanylate cyclase homologue [J].

Gray, JM ;

Karow, DS ;

Lu, H ;

Chang, AJ ;

Chang, JS ;

Ellis, RE ;

Marletta, MA ;

Bargmann, CI .

NATURE, 2004, 430 (6997) :317-322

[8]

Sickle cell vasoocclusion and rescue in a microfluidic device [J].

Higgins, J. M. ;

Eddington, D. T. ;

Bhatia, S. N. ;

Mahadevan, L. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (51) :20496-20500

[9]

Universal microfluidic gradient generator [J].

Irimia, Daniel ;

Geba, Dan A. ;

Toner, Mehmet .

ANALYTICAL CHEMISTRY, 2006, 78 (10) :3472-3477

[10]

Generation of solution and surface gradients using microfluidic systems [J].

Jeon, NL ;

Dertinger, SKW ;

Chiu, DT ;

Choi, IS ;

Stroock, AD ;

Whitesides, GM .

LANGMUIR, 2000, 16 (22) :8311-8316

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