Chemical tools for understanding protein lipidation in eukaryotes

被引：52

作者：

Charron, Guillaume ^{[1
]}

Wilson, John ^{[1
]}

Hang, Howard C. ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Chem Biol & Microbial Pathogenesis, New York, NY 10065 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2009年 / 13卷 / 04期

关键词：

GDP-DISSOCIATION INHIBITOR; N-MYRISTOYL TRANSFERASE; IN-VITRO; FARNESYL DIPHOSPHATE; ANCHORED PROTEINS; MAMMALIAN-CELLS; RAPID DETECTION; WNT PROTEIN; FATTY-ACIDS; PALMITOYLATION;

D O I：

10.1016/j.cbpa.2009.07.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipidation of proteins is an important mechanism to regulate protein trafficking and activity in cell and tissues. The targeting of proteins to membranes by lipidation plays key roles in many physiological processes and when not regulated properly can lead to cancer and neurological disorders. Dissecting the precise roles of protein lipidation in physiology and disease is a major challenge. Recent advances in chemical biology have now enabled the semisynthesis of lipidated proteins for fundamental biochemical and cellular studies. In addition, new chemical reporters of protein lipidation have improved the detection and enabled the proteomic analysis of lipidated proteins. The expanding efforts in chemical biology are therefore providing new tools to dissect the mechanisms and functions of protein lipidation as well as develop therapeutics targeted at protein lipidation pathways in disease.

引用

页码：382 / 391

页数：10

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