Shape and compaction of Escherichia coli nucleoids

被引：82

作者：

Zimmerman, Steven B. ^{[1
]}

机构：

[1] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2006年 / 156卷 / 02期

关键词：

bacteria; chloramphenicol; DNA-binding proteins; DNA condensation; Escherichia coli; doublet; macromolecular crowding; mecillinam; nucleoid; phase; polyamines; puromycin; spermidine; toroid;

D O I：

10.1016/j.jsb.2006.03.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genomic DNA in cells of Escherichia coli is localized in one or two compact, phase-like regions with characteristic shapes. Nucleoids undergo progressive changes in shape and compaction in the presence of drugs such as chloramphenicol or puromycin. Forces which influence nucleoid shape and compaction are reviewed, with particular emphasis on crowding effects of the cytoplasm and confinement effects of the cell envelope. Based in part on the theory of Kornyshev and Leikin for interaction between DNA duplexes, the folding of DNA caused by binding of DNA-associated proteins is suggested to antagonize DNA condensation and, thereby, increase access to DNA sequences. These views are incorporated into a working model for nucleoid organization. Published by Elsevier Inc.

引用

页码：255 / 261

页数：7

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