RETRACTED: Reactome Array: Forging a Link Between Metabolome and Genome (Retracted article. See vol. 330, pg. 912, 2010)

被引：30

作者：

Beloqui, Ana ^{[3
]}

Guazzaroni, Maria-Eugenia ^{[3
]}

Pazos, Florencio ^{[2
]}

Vieites, Jose M. ^{[3
]}

Godoy, Marta ^{[2
]}

Golyshina, Olga V. ^{[1
]}

Chernikova, Tatyana N. ^{[1
]}

Waliczek, Agnes ^{[1
]}

Silva-Rocha, Rafael ^{[2
]}

Al-ramahi, Yamal ^{[3
]}

La Cono, Violetta ^{[4
]}

Mendez, Carmen ^{[5
]}

Salas, Jose A. ^{[5
]}

Solano, Roberto ^{[2
]}

Yakimov, Michail M. ^{[4
]}

Timmis, Kenneth N. ^{[1
,6
]}

Golyshin, Peter N. ^{[1
,7
,8
]}

Ferrer, Manuel ^{[3
]}

机构：

[1] HZI Helmholtz Ctr Infect Res, D-38124 Braunschweig, Germany

[2] CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain

[3] CSIC, Inst Catalysis, E-28049 Madrid, Spain

[4] CNR, Inst Coastal Marine Environm, I-98122 Messina, Italy

[5] Univ Oviedo, E-33006 Oviedo, Spain

[6] Tech Univ Carolo Wilhelmina Braunschweig, Inst Microbiol, D-38106 Braunschweig, Germany

[7] Bangor Univ, Sch Biol Sci, Bangor LL57 2UW, Gwynedd, Wales

[8] Aberystwyth Univ Bangor Univ Partnership CIRRE, Ctr Integrated Res Rural Environm, Aberystwyth, Dyfed, Wales

来源：

SCIENCE | 2009年 / 326卷 / 5950期

关键词：

METAGENOMICS; PATHWAYS; ARCHAEA; STRESS;

D O I：

10.1126/science.1174094

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a sensitive metabolite array for genome sequence-independent functional analysis of metabolic phenotypes and networks, the reactomes, of cell populations and communities. The array includes 1676 dye-linked substrate compounds collectively representing central metabolic pathways of all forms of life. Application of cell extracts to the array leads to specific binding of enzymes to cognate substrates, transformation to products, and concomitant activation of the dye signals. Proof of principle was shown by reconstruction of the metabolic maps of model bacteria. Utility of the array for unsequenced organisms was demonstrated by reconstruction of the global metabolisms of three microbial communities derived from acidic volcanic pool, deep-sea brine lake, and hydrocarbon-polluted seawater. Enzymes of interest are captured on nanoparticles coated with cognate metabolites, sequenced, and their functions unequivocally established.

引用

页码：252 / 257

页数：6

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