Operator-induced errors in Hartmann-Shack wavefront sensing: Model eye study

PURPOSE: To evaluate the effects of instrument realignment and angular misalignment during the clinical determination of wavefront aberrations by simulation in model eyes. SETTING: Aston Academy of Life Sciences, Aston University, Birmingham, United Kingdom. METHODS: Six model eyes were examined with wavefront-aberration-supported cornea ablation (WASCA) (Carl Zeiss Meditec) in 4 sessions of 10 measurements each: sessions 1 and 2, consecutive repeated measures without realignment; session 3, realignment of the instrument between readings; session 4, measurements without realignment but with the model eye shifted 6 degrees angularly. Intersession repeatability and the effects of realignment and misalignment were obtained by comparing the measurements in the various sessions for coma, spherical aberration, and higher-order aberrations (HOAs). RESULTS: The mean differences between the 2 sessions without realignment of the instrument were 0.020 mu m +/- 0.076 (SD) for Z(3)(-1)(P =.551),0.009 +/- 0.139 mu m for Z(3)(1) (P=.877),0.004 +/- 0.037 mu m for Z(4)(0) (P =.820), and 0.005 + 0.01 mu m for HO root mean square (RMS) (P=.301). Differences between the nonrealigned and realigned instruments were -0.017 +/- 0.026 mu m for Z(3)(-1) (P =.159),0.009 +/- 0.028 mu m for Z(3)(1) (P =.475), 0.007 +/- 0.014 mu m for Z(4)(0)(P =.296), and 0.002 +/- 0.007 mu m for HO RMS (P = 0.529; differences between centered and misaligned instruments were -0.355 +/- 0.149 mu m for Z(3)(-1) (P=.002), 0.007 +/- 0.034 mu m for Z(3)(1) (P =.620), -0.005 +/- 0.081 mu m for Z(4)(0)(P =.885), and 0.012 +/- 0.020 mu m for HO RMS (P =.195). Realignment increased the standard deviation by a factor of 3 compared with the first session without realignment. CONCLUSIONS: Repeatability of the WASCA was excellent in all situations tested. Realignment substantially increased the variance of the measurements. Angular misalignment can result in significant errors, particularly in the determination of coma. These findings are important when assessing highly aberrated eyes during follow-up or before surgery.