Hybrid hydrogel-aligned carbon nanotube scaffolds to enhance cardiac differentiation of embryoid bodies

被引：134

作者：

Ahadian, Samad ^{[1
]}

Yamada, Shukuyo ^{[2
]}

Ramon-Azcon, Javier ^{[3
,4
]}

Estili, Mehdi ^{[5
]}

Liang, Xiaobin ^{[1
]}

Nakajima, Ken ^{[1
]}

Shiku, Hitoshi ^{[2
]}

Khademhosseini, Ali ^{[1
,6
,7
,8
,9
,10
]}

Matsue, Tomokazu ^{[1
,2
]}

机构：

[1] Tohoku Univ, WPI Adv Inst Mat Res, Sendai, Miyagi 9808577, Japan

[2] Tohoku Univ, Grad Sch Environm Studies, Sendai, Miyagi 9808579, Japan

[3] IQAC CSIC, Nanobiotechnol Diagnost Grp Nb4D, Jordi Girona 18-26, Barcelona 08034, Spain

[4] CIBER Bioingn Biomat & Nanomed CIBER BBN, Jordi Girona 18-26, Barcelona 08034, Spain

[5] Natl Inst Mat Sci, Mat Proc Unit, Adv Ceram Grp, Tsukuba, Ibaraki 3050047, Japan

[6] Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Med,Ctr Biomed Engn, Cambridge, MA 02139 USA

[7] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[8] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[9] Konkuk Univ, Dept Bioind Technol, Coll Anim Biosci & Technol, Seoul 143701, South Korea

[10] King Abdulaziz Univ, Fac Sci, Dept Phys, Jeddah 21569, Saudi Arabia

来源：

ACTA BIOMATERIALIA | 2016年 / 31卷

关键词：

Carbon nanotubes; Cardiac differentiation; Electrical stimulation; Embryoid body; Hydrogel; MESENCHYMAL STEM-CELLS; ELECTRICAL-STIMULATION; TISSUE; GENERATION;

D O I：

10.1016/j.actbio.2015.11.047

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Carbon nanotubes (CNTs) were aligned in gelatin methacryloyl (GelMA) hydrogels using dielectrophoresis approach. Mouse embryoid bodies (EBs) were cultured in the microwells fabricated on the aligned CNT-hydrogel scaffolds. The GelMA-dielectrophoretically aligned CNT hydrogels enhanced the cardiac differentiation of the EBs compared with the pure GelMA and GelMA-random CNT hydrogels. This result was confirmed by Troponin-T immunostaining, the expression of cardiac genes (i.e., Tnnt2, Nkx2-5, and Actc1), and beating analysis of the EBs. The effect on EB properties was significantly enhanced by applying an electrical pulse stimulation (frequency, 1 Hz; voltage, 3 V; duration, 10 ms) to the EBs for two continuous days. Taken together, the fabricated hybrid hydrogel-aligned CNT scaffolds with tunable mechanical and electrical characteristics offer an efficient and controllable platform for electrically induced differentiation and stimulation of stem cells for potential tissue regeneration and cell therapy applications. Statement of significance Dielectrophoresis approach was used to rapidly align carbon nanotubes (CNTs) in gelatin methacryloyl (GelMA) hydrogels resulting in hybrid GelMA-CNT hydrogels with tunable and anisotropic electrical and mechanical properties. The GelMA-aligned CNT hydrogels may be used to apply accurate and controllable electrical pulses to cell and tissue constructs and thereby regulating their behavior and function. In this work, it was demonstrated that the GelMA hydrogels containing the aligned CNTs had superior performance in cardiac differentiation of stem cells upon applying electrical stimulation in contrast with control gels. Due to broad use of electrical stimulation in tissue engineering and stem cell differentiation, it is envisioned that the GelMA-aligned CNT hydrogels would find wide applications in tissue regeneration and stem cell therapy. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：134 / 143

页数：10

共 46 条

[21]

Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts [J].