Genome-wide microRNA profiling in human fetal nervous tissues by oligonucleotide microarray

被引：55

作者：

Zhao, Jian-Jun

Hua, You-Jia

Sun, Da-Guang

Meng, Xian-Xin

Xiao, Hua-Sheng

Ma, Xu ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Dept Genet, Beijing 10005, Peoples R China

[2] Peking Union Med Coll, Beijing 10005, Peoples R China

[3] Natl Res Inst Family Planning, Dept Genet, Beijing 100081, Peoples R China

[4] Natl Engn Ctr Biochip Shangahai, Shanghai 201203, Peoples R China

[5] Chinese Acad Sci, Shanghai Inst Biol Sci, Bioinformat Ctr, Shanghai 200031, Peoples R China

来源：

CHILDS NERVOUS SYSTEM | 2006年 / 22卷 / 11期

关键词：

microRNA; oligonucleotide microarray; expression profile; nervous system development;

D O I：

10.1007/s00381-006-0173-9

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Objects Our objective was to develop an oligonucleotide DNA microarray (OMA) for genome-wide microRNA profiling and use this method to find miRNAs, which control organic development especially for nervous system. Materials and methods Eighteen organic samples included cerebrum and spinal cord samples from two aborted human fetuses. One was 12 gestational weeks old (G12w) and the other was 24 gestational weeks old (G24w). Global miRNA expression patterns of different organs were investigated using OMA and Northern blot. Conclusion The OMA revealed that 72-83% of miRNAs were expressed in human fetal organs. A series of microRNAs were found specifically and higher-expressed in the human fetal nervous system and confirmed consistently by Northern blot, which may play a critical role in nervous system development.

引用

页码：1419 / 1425

页数：7

共 17 条

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