Disposable microfluidic ELISA for the rapid determination of folic acid content in food products

被引：120

作者：

Hoegger, Daniela ^{[1
]}

Morier, Patrick ^{[1
]}

Vollet, Christine ^{[1
]}

Heini, Dominique ^{[1
]}

Reymond, Frederic ^{[1
]}

Rossier, Joeel S. ^{[1
]}

机构：

[1] DiagnoSwiss SA, Cimo SA CP, CH-1870 Monthey, Switzerland

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 387卷 / 01期

关键词：

electroanalytical methods; bioassays; immunoassays; ELISA; biosensors; enzymes; microfluidics; microfabrication;

D O I：

10.1007/s00216-006-0948-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A micro-analytical system for rapid and quantitative analysis by inhibition immunoassay is presented and applied to the detection of folic acid. Eight polymer microchannels of 65-nL volume each and containing microelectrodes are embedded in a cartridge so that they can be operated simultaneously. All fluidic steps as well as the amperometric detection in the channels are operated by an instrument and software developed in-house. The fluidic steps of the immunoassay occur through hydrodynamic loading of the different solutions through the channels. The speed and duration of the flow and incubation parameters can thus be adapted to the biological and testing requirements. The effectiveness of the system was demonstrated by analysing folic acid concentrations in real infant formula samples within 5 min. In an effort to get a fully monitored assay, each fluidic step is monitored thanks to continuous amperometric detection of oxygen in the microchannel.

引用

页码：267 / 275

页数：9

共 28 条

[1] COMPUTER INTERFACED FAST ANALYZERS [J].

ANDERSON, NG .

SCIENCE, 1969, 166 (3903) :317-&

[2] Development of a multichannel microfluidic analysis system employing affinity capillary electrophoresis for immunoassay [J].

Cheng, SB ;

Skinner, CD ;

Taylor, J ;

Attiya, S ;

Lee, WE ;

Picelli, G ;

Harrison, DJ .

ANALYTICAL CHEMISTRY, 2001, 73 (07) :1472-1479

[3] Microchip-based capillary electrophoresis for immunoassays: Analysis of monoclonal antibodies and theophylline [J].

Chiem, N ;

Harrison, DJ .

ANALYTICAL CHEMISTRY, 1997, 69 (03) :373-378

[4]

Chiem NH, 1998, CLIN CHEM, V44, P591

[5] Application of microchip assay system for the measurement of C-reactive protein in human saliva [J].

Christodoulides, N ;

Mohanty, S ;

Miller, CS ;

Langub, MC ;

Floriano, PN ;

Dharshan, P ;

Ali, MF ;

Bernard, B ;

Romanovicz, D ;

Anslyn, E ;

Fox, PC ;

McDevitt, JT .

LAB ON A CHIP, 2005, 5 (03) :261-269

[6] Electrokinetically driven microfluidic chips with surface-modified chambers for heterogeneous immunoassays [J].

Dodge, A ;

Fluri, K ;

Verpoorte, E ;

de Rooij, NF .

ANALYTICAL CHEMISTRY, 2001, 73 (14) :3400-3409

[7] Development of a novel electrokinetically driven microfluidic immunoassay for the detection of Helicobacter pylori [J].

Gao, YL ;

Lin, FY ;

Hu, GQ ;

Sherman, PA ;

Li, DQ .

ANALYTICA CHIMICA ACTA, 2005, 543 (1-2) :109-116

[8] Automated microarray system for the simultaneous detection of antibiotics in milk [J].

Knecht, BG ;

Strasser, A ;

Dietrich, R ;

Märtlbauer, E ;

Niessner, R ;

Weller, MG .

ANALYTICAL CHEMISTRY, 2004, 76 (03) :646-654

[9] Design of a compact disk-like microfluidic platform for enzyme-linked immunosorbent assay [J].

Lai, S ;

Wang, SN ;

Luo, J ;

Lee, LJ ;

Yang, ST ;

Madou, MJ .

ANALYTICAL CHEMISTRY, 2004, 76 (07) :1832-1837

[10] Magnetically actuated micromixing on an array-pattern microfluidic chip for immunoassay of human thyrotropin [J].

Li, CW ;

Wang, YN ;

Gao, YH ;

Guo, XY ;

Gu, ZZ .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2005, 5 (08) :1297-1300

← 1 2 3 →