Flow injection analysis using continuous channel electrophoresis

被引:21
作者
Mesaros, JM [1 ]
Gavin, PF [1 ]
Ewing, AG [1 ]
机构
[1] PENN STATE UNIV,DEPT CHEM,DAVEY LAB 152,UNIVERSITY PK,PA 16802
关键词
D O I
10.1021/ac9601371
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Continuous zone electrophoretic separations in narrow channels coupled to small-bore capillaries have been demonstrated and characterized previously. Presented here is the use of this new technique to monitor dynamic chemical changes occurring in a flow injection analysis system, The fundamental aspects of the data that this type of separation generates are discussed in a comparison of static and dynamic analyses, An analysis of a dynamic separation is also provided to thoroughly outline the steps necessary to deconvolute the data, Three types of dynamic analyses, which simulate realistic analytical situations, are then examined in detail, The first of these involves the addition of a mixture of four dansylated amino acids to the flow injection system to provide several different sample duration periods, The second is the staggered addition of short-duration plugs of analyte to the system, The third is the continuous addition of one analyte at different concentrations. Quantitative information generated by these experiments includes the simultaneous determination of the time of analyte contact and its duration of contact with the sampling capillary, identification of analytes based on electrophoretic mobilities, and concentration changes with time, For the addition of a 1.52 mM solution of N-epsilon-dansyl-L-lysine, the sample duration and time of analyte contact with the capillary was determined with an error of <4%. Concentration changes in the FIA system of dansyl-L-arginine over the range of 0.38 -3.04 mM are also demonstrated with transitional edges on the time scale of 3-4 s. Qualitatively, the appearance of analyte bands can reveal impurities and fronting or tailing effects as seen in conventional capillary electrophoretic separations, The results demonstrate the ability of the technique to successfully probe dynamic environments.
引用
收藏
页码:3441 / 3449
页数:9
相关论文
共 12 条
[1]   CAPILLARY ELECTROPHORESIS [J].
EWING, AG ;
WALLINGFORD, RA ;
OLEFIROWICZ, TM .
ANALYTICAL CHEMISTRY, 1989, 61 (04) :A292-+
[2]   ELECTROOSMOTIC FLOW-CONTROL AND SURFACE CONDUCTANCE IN CAPILLARY ZONE ELECTROPHORESIS [J].
HAYES, MA ;
KHETERPAL, I ;
EWING, AG .
ANALYTICAL CHEMISTRY, 1993, 65 (15) :2010-2013
[3]   CAPILLARY ZONE ELECTROPHORESIS [J].
JORGENSON, JW ;
LUKACS, KD .
SCIENCE, 1983, 222 (4621) :266-272
[4]   ZONE ELECTROPHORESIS IN OPEN-TUBULAR GLASS-CAPILLARIES [J].
JORGENSON, JW ;
LUKACS, KD .
ANALYTICAL CHEMISTRY, 1981, 53 (08) :1298-1302
[5]  
KUHR WG, 1990, ANAL CHEM, V62, pR403, DOI 10.1021/ac00211a022
[6]   NANOLITER VOLUME KINETIC ASSAYS [J].
LIU, YM ;
SWEEDLER, JV .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (34) :8871-8872
[7]   CAPILLARY ZONE ELECTROPHORESIS - EFFECT OF PHYSICAL PARAMETERS ON SEPARATION EFFICIENCY AND QUANTITATION [J].
LUKACS, KD ;
JORGENSON, JW .
JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1985, 8 (08) :407-411
[8]   CHARACTERIZATION OF CONTINUOUS ELECTROPHORETIC SEPARATIONS IN NARROW CHANNELS COUPLED TO SMALL-BORE CAPILLARIES [J].
MESAROS, JM ;
EWING, AG .
JOURNAL OF MICROCOLUMN SEPARATIONS, 1994, 6 (05) :483-494
[9]   CONTINUOUS ELECTROPHORETIC SEPARATIONS IN NARROW CHANNELS COUPLED TO SMALL-BORE CAPILLARIES [J].
MESAROS, JM ;
LUO, G ;
ROERAADE, J ;
EWING, AG .
ANALYTICAL CHEMISTRY, 1993, 65 (22) :3313-3319
[10]   HIGH-PERFORMANCE ZONE ELECTROPHORESIS [J].
MIKKERS, FEP ;
EVERAERTS, FM ;
VERHEGGEN, TPEM .
JOURNAL OF CHROMATOGRAPHY, 1979, 169 (FEB) :11-20