Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds

被引：65

作者：

Arecchi, Alessandra ^{[1
]}

Scampicchio, Matteo ^{[1
]}

Drusch, Stephan ^{[2
]}

Mannino, Saverio ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Sci & Tecnol Alimentari, I-20133 Milan, Italy

[2] Univ Kiel, Food Technol Div, D-24118 Kiel, Germany

来源：

ANALYTICA CHIMICA ACTA | 2010年 / 659卷 / 1-2期

关键词：

Phenols; Biosensor; Nanofiber; Electrospinning; Nylon; Tyrosinase; ELECTROCHEMICAL DETECTION; CARBON NANOTUBE; POLYPHENOLS; OXIDATION; CATECHOL; LIQUID; ACID; FILM;

D O I：

10.1016/j.aca.2009.11.039

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A tyrosinase-modified electrode is described to be used as amperometric biosensor for the detection of phenolic compounds in food. The enzyme has been immobilized by drop-coating on a glassy carbon electrode covered by a polyamidic nanofibrous membrane prepared by electrospinning. With respect to others, the selectivity of the designed tyrosinase-biosensor resulted modified by the presence of the nanostructured coating which seems to affect the permeability of phenols as a function of the pH of the solution and of their dissociation constants. The biosensor exhibits a response time of 16 s, a detection limit of 0.05 mu M, and a linearity up to 100 mu M (slope: -304 nA mu M-1; intercept: -191 nA. r(2) = 0.996, n = 19). Among others. it can be successfully used for monitoring in real time the release kinetics of phenols encapsulated in polymeric microcapsules. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：133 / 136

页数：4

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