Human mini-chromosomes in mouse embryonal stem cells

被引：54

作者：

Shen, MH

Yang, J

Loupart, ML

Smith, A

Brown, W

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,CANC RES CAMPAIGN,CHROMOSOME MOL BIOL GRP,OXFORD OX1 3QU,ENGLAND

[2] UNIV EDINBURGH,CTR GENOME RES,EDINBURGH EH9 3JQ,MIDLOTHIAN,SCOTLAND

来源：

HUMAN MOLECULAR GENETICS | 1997年 / 6卷 / 08期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/hmg/6.8.1375

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have introduced human mini-chromosomes of 4 Mb and similar to 15 Mb in size into mouse embryonal stem cells, Although these human mini-chromosomes are stable in hamster and chicken cells, they re-arrange or segregate aberrantly in the embryonal stem cells and are rapidly lost in the absence of selection, However, one of the mini-chromosomes re-arranged, acquired mouse centromeric sequences and was then stably maintained for at least 60 population doublings in culture, This mini-chromosome, which is 4 Mb in size, is a candidate for a mouse germ line chromosome vector.

引用

页码：1375 / 1382

页数：8

共 15 条

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