Floating electrode dielectric barrier discharge plasma in air promoting apoptotic behavior in melanoma skin cancer cell lines

被引:483
作者
Fridman, Gregory
Shereshevsky, Alexey
Jost, Monika M.
Brooks, Ari D.
Fridman, Alexander
Gutsol, Alexander
Vasilets, Victor
Friedman, Gary
机构
[1] Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USA
[2] Drexel Univ, Coll Med, Dept Surg, Philadelphia, PA 19102 USA
[3] Drexel Univ, Coll Med, Dept Radiat Oncol, Philadelphia, PA 19102 USA
[4] Drexel Univ, Dept Mech Engn & Mech, Philadelphia, PA 19104 USA
[5] Drexel Univ, Dept Elect & Comp Engn, Philadelphia, PA 19130 USA
基金
美国国家科学基金会;
关键词
non-thermal plasma; dielectric barrier discharges (DBDs); apoptosis; melanoma cancer cells; cancer treatment; skin diseases; SURFACE MODIFICATION; GLOW-DISCHARGE; AVALANCHE KNIFE; STERILIZATION; COAGULATION; ELECTROCAUTERY; BACTERIA; DECONTAMINATION; SAFETY; ENERGY;
D O I
10.1007/s11090-007-9048-4
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
Initiation of apoptosis, or programmed cell death, is an important issue in cancer treatment as cancer cells frequently have acquired the ability to block apoptosis and thus are more resistant to chemotherapeutic drugs. Targeted and perhaps selective destruction of cancerous tissue is desirable for many reasons, ranging from the enhancement of or aid to current medical methods to problems currently lacking a solution, i.e., lung cancer. Demonstrated in this publication is the inactivation (killing) of human Melanoma skin cancer cell lines, in vitro, by Floating Electrode Dielectric Barrier Discharge (FE-DBD) plasma. Not only are these cells shown to be killed immediately by high doses of plasma treatment, but low doses are shown to promote apoptotic behavior as detected by TUNEL staining and subsequent flow cytometry. It is shown that plasma acts on the cells directly and not by "poisoning" the solution surrounding the cells, even through a layer of such solution. Potential mechanisms of interaction of plasma with cells are discussed and further steps are proposed to develop an understanding of such systems.
引用
收藏
页码:163 / 176
页数:14
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