Generation of Functional Ventricular Heart Muscle from Mouse Ventricular Progenitor Cells

被引：152

作者：

Domian, Ibrahim J. ^{[1
,2
]}

Chiravuri, Murali ^{[1
]}

van der Meer, Peter ^{[1
,3
]}

Feinberg, Adam W. ^{[4
]}

Shi, Xi ^{[1
]}

Shao, Ying ^{[1
]}

Wu, Sean M. ^{[1
,2
]}

Parker, Kevin Kit ^{[2
,4
,5
]}

Chien, Kenneth R. ^{[1
,2
,6
]}

机构：

[1] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Boston, MA 02114 USA

[2] Harvard Stem Cell Inst, Cambridge, MA 02138 USA

[3] Univ Groningen, Univ Med Ctr Groningen, Dept Cardiol, NL-9700 RB Groningen, Netherlands

[4] Harvard Univ, Sch Engn & Appl Sci, Dis Biophys Grp, Cambridge, MA 02138 USA

[5] Harvard Univ, Wyss Inst Biologically Inspired Engn, Boston, MA 02115 USA

[6] Harvard Univ, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

来源：

SCIENCE | 2009年 / 326卷 / 5951期

关键词：

SMOOTH-MUSCLE; ISL1; DIFFERENTIATION; TRANSCRIPTION; EXPRESSION; MEF2C;

D O I：

10.1126/science.1177350

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mammalian heart is formed from distinct sets of first and second heart field (FHF and SHF, respectively) progenitors. Although multipotent progenitors have previously been shown to give rise to cardiomyocytes, smooth muscle, and endothelial cells, the mechanism governing the generation of large numbers of differentiated progeny remains poorly understood. We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic stem cells. Genome-wide profiling of coding and noncoding transcripts revealed distinct molecular signatures of these progenitor populations. We further identify a committed ventricular progenitor cell in the Islet 1 lineage that is capable of limited in vitro expansion, differentiation, and assembly into functional ventricular muscle tissue, representing a combination of tissue engineering and stem cell biology.

引用

页码：426 / 429

页数：4

共 21 条

[1] The Snail genes as inducers of cell movement and survival: implications in development and cancer [J].

Barrallo-Gimeno, A ;

Nieto, MA .

DEVELOPMENT, 2005, 132 (14) :3151-3161

[2] Snail1a and Snail1b cooperate in the anterior migration of the axial mesendoderm in the zebrafish embryo [J].

Blanco, Maria J. ;

Barrallo-Gimeno, Alejandro ;

Acloque, Herve ;

Reyes, Ariel E. ;

Tada, Masazumi ;

Allende, Miguel L. ;

Mayor, Roberto ;

Nieto, M. Angela .

DEVELOPMENT, 2007, 134 (22) :4073-4081

[3] A murine model of Holt-Oram syndrome defines roles of the T-box transcription factor Tbx5 in cardiogenesis and disease [J].

Bruneau, BG ;

Nemer, G ;

Schmitt, JP ;

Charron, F ;

Robitaille, L ;

Caron, S ;

Conner, DA ;

Gessler, M ;

Nemer, M ;

Seidman, CE ;

Seidman, JG .

CELL, 2001, 106 (06) :709-721

[4] Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages [J].

Bu, Lei ;

Jiang, Xin ;

Martin-Puig, Silvia ;

Caron, Leslie ;

Zhu, Shenjun ;

Shao, Ying ;

Roberts, Drucilla J. ;

Huang, Paul L. ;

Domian, Ibrahim J. ;

Chien, Kenneth R. .

NATURE, 2009, 460 (7251) :113-U130

[5] Building the mammalian heart from two sources of myocardial cells [J].

Buckingham, M ;

Meilhac, S ;

Zaffran, S .

NATURE REVIEWS GENETICS, 2005, 6 (11) :826-835

[6] Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart [J].

Cai, CL ;

Liang, XQ ;

Shi, YQ ;

Chu, PH ;

Pfaff, SL ;

Chen, J ;

Evans, S .

DEVELOPMENTAL CELL, 2003, 5 (06) :877-889

[7] Mef2c is a direct transcriptional target of ISL1 and GATA factors in the anterior heart field during mouse embryonic development [J].

Dodou, E ;

Verzi, MP ;

Anderson, JR ;

Xu, SM ;

Black, BL .

DEVELOPMENT, 2004, 131 (16) :3931-3942

[8] mef2c is activated directly by myogenic basic helix-loop-helix proteins during skeletal muscle development in vivo [J].

Dodou, E ;

Xu, SM ;

Black, BL .

MECHANISMS OF DEVELOPMENT, 2003, 120 (09) :1021-1032

[9] Muscular thin films for building actuators and powering devices [J].

Feinberg, Adam W. ;

Feigel, Alex ;

Shevkoplyas, Sergey S. ;

Sheehy, Sean ;

Whitesides, George M. ;

Parker, Kevin Kit .

SCIENCE, 2007, 317 (5843) :1366-1370

[10] Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency [J].

Hanna, Jacob ;

Markoulaki, Styliani ;

Schorderet, Patrick ;

Carey, Bryce W. ;

Beard, Caroline ;

Wernig, Marius ;

Creyghton, Menno P. ;

Steine, Eveline J. ;

Cassady, John P. ;

Foreman, Ruth ;

Lengner, Christopher J. ;

Dausman, Jessica A. ;

Jaenisch, Rudolf .

CELL, 2008, 133 (02) :250-264

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