High-purity magnesium interference screws promote fibrocartilaginous entheses regeneration in the anterior cruciate ligament reconstruction rabbit model via accumulation of BMP-2 and VEGF

被引：160

作者：

Cheng, Pengfei ^{[1
]}

Han, Pei ^{[1
]}

Zhao, Changli ^{[2
]}

Zhang, Shaoxiang ^{[2
,3
]}

Wu, Hongliu ^{[2
]}

Ni, Jiahua ^{[2
]}

Hou, Peng ^{[1
]}

Zhang, Yuanzhuang ^{[3
]}

Liu, Jingyi ^{[3
]}

Xu, Haidong ^{[3
]}

Liu, Shen ^{[1
]}

Zhang, Xiaonong ^{[2
,3
]}

Zheng, Yufeng ^{[4
]}

Chai, Yimin ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthopaed, Shanghai 200233, Peoples R China

[2] Shanghai Jiao Tong Univ, State Key Lab Met Matrix Composites, Sch Mat Sci & Engn, Shanghai 200240, Peoples R China

[3] Suzhou Origin Med Technol Co Ltd, Suzhou 215513, Peoples R China

[4] Peking Univ, Dept Mat Sci & Engn, Coll Engn, Beijing 100871, Peoples R China

来源：

BIOMATERIALS | 2016年 / 81卷

关键词：

High-purity magnesium; Interference screw; Anterior cruciate ligament reconstruction; Fibrocartilaginous entheses; BMP-2; VEGF; ENDOTHELIAL GROWTH-FACTOR; TO-BONE INTERFACE; RAT MODEL; IN-VITRO; STROMAL CELLS; TENDON GRAFT; TUNNEL; IMPLANTATION; DEGRADATION; EXPRESSION;

D O I：

10.1016/j.biomaterials.2015.12.005

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Interference screw in the fixation of autologous tendon graft to the bone tunnel is widely accepted for the reconstruction of anterior cruciate ligament (ACL), but the regeneration of fibrocartilaginous entheses could hardly be achieved with the traditional interference screw. In the present work, biodegradable high-purity magnesium (HP Mg) showed good cytocompatibility and promoted the expression of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF), fibrocartilage markers (Aggrecan, COL2A1 and SOX-9), and glycosaminoglycan (GAG) production in vitro. The HP Mg screw was applied to fix the semitendinosus autograft to the femoral tunnel in a rabbit model of ACL reconstruction with titanium (Ti) screw as the control. The femur-tendon graft-tibia complex was retrieved at 3, 6, 9 and 12 weeks. Gross observation and range of motion (ROM) of the animal model reached normal levels at 12 weeks. No sign of host reaction was found in the X-ray scanning. The HP Mg group was comparable to the Ti group with respect to biomechanical properties of the reconstructed ACL, and the ultimate load to failure and stiffness increased 12 weeks after surgery. In the histological analysis, the HP Mg group formed distinct fibrocartilage transition zones at the tendon-bone interface 12 weeks after surgery, whereas a disorganized fibrocartilage layer was found in the Ti group. In the immunohistochemical analysis, highly positive staining of BMP-2, VEGF and the specific receptor for BMP-2 (BMPRIA) was shown at the tendon-bone interface of the HP Mg group compared with the Ti group. Furthermore, the HP Mg group had significantly higher expression of BMP-2 and VEGF than the Ti group in the early phase of tendon-bone healing, followed by enhanced expression of fibrocartilage markers and GAG production. Therefore we proposed that the stimulation of BMP-2 and VEGF by Mg ions was responsible for the fibrochondrogenesis of Mg materials. HP Mg was promising as a biodegradable interference screw with the potential to promote fibrocartilaginous entheses regeneration in ACL reconstruction. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：14 / 26

页数：13

共 48 条

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Characterization of the structure-function relationship at the ligament-to-bone interface [J].