Cell-type and donor-specific transcriptional responses to interferon-α -: Use of customized gene arrays

被引:70
作者
Schlaak, JF
Hilkens, CMU
Costa-Pereira, AP
Strobl, B
Aberger, F
Frischauf, AM
Kerr, IM
机构
[1] Canc Res UK, London Res Inst, London WC2A 3PX, England
[2] Inst Anim Breeding & Genet, A-1210 Vienna, Austria
[3] Salzburg Univ, Genet Inst, A-5020 Salzburg, Austria
关键词
D O I
10.1074/jbc.M205571200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive, specific, reproducible, robust, and cost-effective customized cDNA array system based on established nylon membrane technology has been developed for convenient multisample expression profiling for several hundred genes of choice. The genes represented are easily adjusted (depending on the availability of corresponding cDNAs) and the method is accordingly readily applicable to a wide variety of systems. Here we have focused on the expression profiles for interferon-alpha2a, the most widely used interferon for the treatment of viral hepatitis and malignancies, in primary cells (peripheral blood mononuclear cells, T cells, and dendritic cells) and cell lines (Kit255, HT1080, HepG2, and HuH7). Of 150 genes studied, only six were consistently induced in all cell types and donors, whereas 74 genes were induced in at least one cell type. IRF-7 was identified as the only gene exclusively induced in the hematopoietic cells. No gene was exclusively induced in the nonhematopoietic cell lines. In T cells 12, and in dendritic cells, 25 genes were induced in all donors whereas 45 and 42 genes, respectively, were induced in at least one donor. The data suggest that signaling through IFN-alpha2 can be substantially modulated to yield significant cell-type and donor-specific qualitative and quantitative differences in gene expression in response to this cytokine under highly standardized conditions.
引用
收藏
页码:49428 / 49437
页数:10
相关论文
共 56 条
[51]   Virus infection induces the assembly of coordinately activated transcription factors on the IFN-β enhancer in vivo [J].
Wathelet, MG ;
Lin, CH ;
Parekh, BS ;
Ronco, LV ;
Howley, PM ;
Maniatis, T .
MOLECULAR CELL, 1998, 1 (04) :507-518
[52]  
Whitney LW, 1999, ANN NEUROL, V46, P425
[53]  
Yuen M F, 2001, Lancet Infect Dis, V1, P232, DOI 10.1016/S1473-3099(01)00118-9
[54]   Cutaneous T cell lymphoma: Update of treatment [J].
Zackheim, HZ .
DERMATOLOGY, 1999, 199 (02) :102-105
[55]   IRF-7, a new interferon regulatory factor associated with Epstein-Barr virus latency [J].
Zhang, LW ;
Pagano, JS .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (10) :5748-5757
[56]   Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+hematopoietic stem/progenitor cells [J].
Zhang, QH ;
Ye, M ;
Wu, XY ;
Ren, SX ;
Zhao, M ;
Zhao, CJ ;
Fu, G ;
Shen, Y ;
Fan, HY ;
Lu, G ;
Zhong, M ;
Xu, XR ;
Han, ZG ;
Zhang, JW ;
Tao, J ;
Huang, QH ;
Zhou, J ;
Hu, GX ;
Gu, J ;
Chen, SJ ;
Chen, Z .
GENOME RESEARCH, 2000, 10 (10) :1546-1560