Nucleolar dominance does not occur in root tip cells of allotetraploid Brassica species

被引：43

作者：

Hasterok, R ^{[1
]}

Maluszynska, J ^{[1
]}

机构：

[1] Silesian Univ, Dept Plant Anat & Cytol, PL-40032 Katowice, Poland

来源：

GENOME | 2000年 / 43卷 / 03期

关键词：

D O I：

10.1139/gen-43-3-574

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Using in situ hybridization and silver staining methods, the numbers of active and inactive rDNA loci have been established for three allotetraploid species of Brassica (B. napus, B. carinata, and B. juncea) and their diploid ancestors (B. campestris, B. nigra, and B. oleracea). The allotetraploid species have chromosome numbers equal to the sum of the numbers in their diploid relatives, but have fewer rDNA loci. All species investigated have lower numbers of active NORs (AgNORs, nucleolar organizer regions) compared with the numbers of rDNA sites revealed by in situ hybridization. The number of active rDNA loci of the allotetraploid species is equal to the number of AgNORs in their diploid ancestors, indicating the absence of nucleolar dominance in amphidiploid Brassica species, at least in root meristematic cells.

引用

页码：574 / 579

页数：6

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