Detection and identification of bacterial cell surface proteins by fluorescent labeling

被引：17

作者：

Anaya, Cecilia

Church, Nadia

Lewis, Janina P.

机构：

[1] Virginia Commonwealth Univ, Sch Dent, Philips Inst Oral & Craniofacial Mol Biol, Richmond, VA 23298 USA

[2] Univ San Luis, San Luis, Argentina

[3] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Richmond, VA USA

来源：

PROTEOMICS | 2007年 / 7卷 / 02期

关键词：

bacteria; fluorescence labeling; mass spectrometry; Porphyromonas gingivalis; surface proteins;

D O I：

10.1002/pmic.200600175

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid method of detection and identification of bacterial cell surface proteins is needed to better understand the interaction of bacteria with host components. To detect cell surface proteins, we have labeled cells of the Gram-negative anaerobic bacterium, Porphyromonas gingivalis, with fluorescent cyanine dyes, Cy3 and Cy5. We demonstrate that only cell surface proteins were labeled, indicating the method applied in our study is suitable for detection and identification of cell surface proteins in Gram-negative bacteria and possibly other organisms.

引用

收藏

页码：215 / 219

页数：5

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