Dynamic electrochemical experiments on hydrogenases

被引:19
作者
Armstrong, Fraser A. [1 ]
机构
[1] Univ Oxford, Inorgan Chem Lab, Dept Chem, Oxford OX1 3QR, England
基金
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;
关键词
Hydrogen; Hydrogenase; Photosynthesis; Electrochemistry; H-2; O-2; UNREADY; READY; INTERCONVERSIONS; INACTIVATION; DIFFERENCE; ACTIVATION; OXIDATION; STATES;
D O I
10.1007/s11120-009-9428-0
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A powerful approach for studying hydrogenases, applying a suite of dynamic electrochemical techniques known as protein film electrochemistry, is trailblazing fresh discoveries and providing a wealth of quantitative data on these complex enzymes. The information now stemming from experiments on tiny quantities of hydrogenases ranges from their kinetics and catalytic bias (a preference to operate in H-2 oxidation vs. H-2 production) to wide differences in the ways they react with oxygen and other inhibitors. Tolerance of hydrogenase catalysis to oxygen is essential if organisms are to be exploited for photosynthetic hydrogen production, and is crucial in enabling aerobes to use trace H-2 as an energy source. Experiments described in this article may be adapted for other complex enzymes.
引用
收藏
页码:541 / 550
页数:10
相关论文
共 24 条
[1]   Dynamic electrochemical investigations of hydrogen oxidation and production by enzymes and implications for future technology [J].
Armstrong, Fraser A. ;
Belsey, Natalie A. ;
Cracknell, James A. ;
Goldet, Gabrielle ;
Parkin, Alison ;
Reisner, Erwin ;
Vincent, Kylie A. ;
Wait, Annemarie F. .
CHEMICAL SOCIETY REVIEWS, 2009, 38 (01) :36-51
[2]   Finding gas diffusion pathways in proteins:: Application to O2 and H2 transport in Cpl [FeFe]-hydrogenase and the role of packing defects [J].
Cohen, J ;
Kim, K ;
King, P ;
Seibert, M ;
Schulten, K .
STRUCTURE, 2005, 13 (09) :1321-1329
[3]   Enzymatic oxidation of H2 in atmospheric O2:: The electrochemistry of energy generation from trace H2 by aerobic microorganisms [J].
Cracknell, James A. ;
Vincent, Kylie A. ;
Ludwig, Marcus ;
Lenz, Oliver ;
Friedrich, Baerbel ;
Armstrong, Fraser A. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (02) :424-+
[4]   Structure/function relationships of [NiFe]- and [FeFe]-hydrogenases [J].
Fontecilla-Camps, Juan C. ;
Volbeda, Anne ;
Cavazza, Christine ;
Nicolet, Yvain .
CHEMICAL REVIEWS, 2007, 107 (10) :4273-4303
[5]   Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms [J].
Ghirardi, Maria L. ;
Posewitz, Matthew C. ;
Maness, Pin-Ching ;
Dubini, Alexandra ;
Yu, Jianping ;
Seibert, Michael .
ANNUAL REVIEW OF PLANT BIOLOGY, 2007, 58 :71-91
[6]   Hydrogen production under aerobic conditions by membrane-bound hydrogenases from Ralstonia species [J].
Goldet, Gabrielle ;
Wait, Annemarie F. ;
Cracknell, James A. ;
Vincent, Kylie A. ;
Ludwig, Marcus ;
Lenz, Oliver ;
Friedrich, Baerbel ;
Armstrong, Fraser A. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (33) :11106-11113
[7]   [FeFe]-Hydrogenase-Catalyzed H2 production in a photoelectrochemical biofuel cell [J].
Hambourger, Michael ;
Gervaldo, Miguel ;
Svedruzic, Drazenka ;
King, Paul W. ;
Gust, Devens ;
Ghirardi, Maria ;
Moore, Ana L. ;
Moore, Thomas A. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (06) :2015-2022
[8]   Direct comparison of the electrocatalytic oxidation of hydrogen by an enzyme and a platinum catalyst [J].
Jones, AK ;
Sillery, E ;
Albracht, SPJ ;
Armstrong, FA .
CHEMICAL COMMUNICATIONS, 2002, (08) :866-867
[9]   The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide [J].
Lamle, SE ;
Albracht, SPJ ;
Armstrong, FA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (18) :6595-6604
[10]   Electrochemical potential-step investigations of the aerobic interconversions of [NiFe]-hydrogenase from Allochromatium vinosum:: Insights into the puzzling difference between unready and ready oxidized inactive states [J].
Lamle, SE ;
Albracht, SPJ ;
Armstrong, FA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (45) :14899-14909