Purification and partial characterization of manganese superoxide dismutase from downy mildew resistant pearl millet seedlings inoculated with Sclerospora graminicola

被引：23

作者：

Babitha, MP ^{[1
]}

Prakash, HS ^{[1
]}

Shetty, HS ^{[1
]}

机构：

[1] Univ Mysore, Dept Studies Appl Bot Seed Pathol & Biotechnol, Downy Mildew Res Lab, Mysore 570006, Karnataka, India

来源：

PLANT SCIENCE | 2002年 / 163卷 / 04期

关键词：

superoxide dismutase; pearl millet; downy mildew; host-pathogen interaction;

D O I：

10.1016/S0168-9452(02)00238-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Mn-SOD was purified from pearl millet by ammonium sulfate precipitation followed by column chromatography using DEAE-cellulose and Sephadex G-100. The isozyme has a molecular weight of 35 kDa. Electrophoresis revealed a single band of SOD activity corresponding to the purified enzyme. The purified enzyme was stable over a pH range of 7.0-9.0 for 24 h, and a temperature range of 20-35 degreesC. The purified pearl millet SOD exhibited insensitivity to hydrogen peroxide and cyanide, which is typical of Mn-containing SOD. SOD was purified 73-fold from pearl millet. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

收藏

页码：917 / 924

页数：8

相关论文

共 35 条

[1] PURIFICATION OF AN IRON-CONTAINING SUPEROXIDE-DISMUTASE FROM A CITRUS PLANT, CITRUS-LIMONUM R [J].

ALMANSA, MS ;

PALMA, JM ;

YANEZ, J ;

DELRIO, LA ;

SEVILLA, F .

FREE RADICAL RESEARCH COMMUNICATIONS, 1991, 12-3 :319-328

[2] Differential induction of superoxide dismutase in downy mildew-resistant and -susceptible genotypes of pearl millet [J].

Babitha, MP ;

Bhat, SG ;

Prakash, HS ;

Shetty, HS .

PLANT PATHOLOGY, 2002, 51 (04) :480-486

[3] ISOLATION AND CHARACTERIZATION OF THE CYTOSOLIC AND MITOCHONDRIAL SUPEROXIDE DISMUTASES OF MAIZE [J].

BAUM, JA ;

SCANDALIOS, JG .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1981, 206 (02) :249-264

[4]

Beauchamp C., 1971, ANAL BIOCHEM, V44, P276, DOI DOI 10.1016/0003-2697(71)90370-8

[5] CHARACTERIZATION OF THE BACILLUS-STEAROTHERMOPHILUS MANGANESE SUPEROXIDE-DISMUTASE GENE AND ITS ABILITY TO COMPLEMENT COPPER-ZINC SUPEROXIDE-DISMUTASE DEFICIENCY IN SACCHAROMYCES-CEREVISIAE [J].

BOWLER, C ;

VANKAER, L ;

VANCAMP, W ;

VANMONTAGU, M ;

INZE, D ;

DHAESE, P .

JOURNAL OF BACTERIOLOGY, 1990, 172 (03) :1539-1546

[6]

BOWLER C, 1994, CRIT REV PLANT SCI, V13, P199, DOI 10.1080/713608062

[7] THE INDUCTION OF MANGANESE SUPEROXIDE-DISMUTASE IN RESPONSE TO STRESS IN NICOTIANA-PLUMBAGINIFOLIA [J].

BOWLER, C ;

ALLIOTTE, T ;

DELOOSE, M ;

VANMONTAGU, M ;

INZE, D .

EMBO JOURNAL, 1989, 8 (01) :31-38

[8]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[9] SOLUBLE SUPEROXIDE-DISMUTASE (SOD) IN SUSCEPTIBLE AND RESISTANT HOST-PARASITE COMPLEXES OF PHASEOLUS-VULGARIS AND UROMYCES-PHASEOLI [J].

BUONAURIO, R ;

DELLATORRE, G ;

MONTALBINI, P .

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1987, 31 (02) :173-184

[10] MECHANISM OF HYPERSENSITIVE CELL COLLAPSE - CORRELATION OF INCREASED LIPOXYGENASE ACTIVITY WITH MEMBRANE DAMAGE IN LEAVES OF PHASEOLUS-VULGARIS (L) INOCULATED WITH AN AVIRULENT RACE OF PSEUDOMONAS-SYRINGAE PV PHASEOLICOLA [J].

CROFT, KPC ;

VOISEY, CR ;

SLUSARENKO, AJ .

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1990, 36 (01) :49-62

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