Modified capillary electrophoresis system for peptide, protein and double-stranded DNA analysis

被引:14
作者
Belenkii, BG [1 ]
Kassalainen, GE [1 ]
Nasledov, DG [1 ]
机构
[1] Russian Acad Sci, Inst Analyt Instrumentat, St Petersburg 198103, Russia
关键词
capillary columns; adsorption; DNA; peptides; proteins; poly(glycidyl methacrylate);
D O I
10.1016/S0021-9673(00)00226-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The results of high-performance capillary electrophoresis (HPCE) studies of peptide, protein and double-stranded DNA separations on a laboratory-made HPCE system are presented. Parameters of the HPCE system are given. The new method of capillary surface modification by grafting poly(glycidyl methacrylate) is described. The problems of HPCE biopolymer analysis connected with the sample-wall interactions are discussed. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:189 / 196
页数:8
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