Purification and properties of an acid phosphatase from Entamoeba histolytica HM-1:IMSS

被引：12

作者：

Aguirre-García, MM

Cerbón, J

Talamás-Rohana, P

机构：

[1] IPN, Inst Res & Adv Studies, Dept Expt Pathol, Mexico City 07360, DF, Mexico

[2] IPN, Ctr Res & Adv Studies, Dept Biochem, Mexico City 07360, DF, Mexico

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2000年 / 30卷 / 05期

关键词：

acid phosphatase; Entamoeba histolytica; parasitic protozoan; pathogenicity; purification; virulence;

D O I：

10.1016/S0020-7519(00)00032-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Entamoeba histolytica contains and secretes acid phosphatase, which has been proposed as a virulence factor in some pathogenic microorganisms. In this work, we purified and characterised a membrane-bound acid phosphatase (MAP) from E. histolytica HM-1:IMSS and studied the effect of different chemical compounds on the secreted acid phosphatase and MAP activities. MAP purification was accomplished by detergent solubilisation, and affinity and ion exchange chromatographies. The enzyme showed a pi of 5.5-6.2, an optimum pH of 5.5, and a Km value of 1.14 mM with p-nitrophenyl phosphate. (C) 2000 Published by Elsevier Science Ltd on behalf of the Australian Society for Parasitology Inc. All rights reserved.

引用

页码：585 / 591

页数：7

共 37 条

[11] PHOSPHONIC AND ARSONIC ACIDS AS INHIBITORS OF HUMAN RED-CELL ACID-PHOSPHATASE AND THEIR USE IN AFFINITY CHROMATOGRAPHY [J].