Resonance light scattering technique for the determination of proteins with resorcinol yellow and OP

被引：57

作者：

Chen, YJ

Yang, JH ^{[1
]}

Wu, X

Wu, T

Luan, YX

机构：

[1] Shandong Univ, Dept Chem, Lab Colloid & Interface Chem, Educ Minist, Jinan 250100, Peoples R China

[2] Jinan Univ, Dept Chem, Jinan 250002, Peoples R China

来源：

TALANTA | 2002年 / 58卷 / 05期

关键词：

OP; resorcinol yellow; protein; resonance light scattering (RLS);

D O I：

10.1016/S0039-9140(02)00401-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new resonance light scattering (RLS) assay of proteins is presented. In the citric acid-NaOH (pH 2.35) buffer, the RLS of Resorcinol yellow (RY)-protein system can be greatly enhanced by addition of nonionic surfactant OP, owing to the interaction between OP and RY-protein. The enhanced RLS is in proportion to the concentration of proteins in the range 0.02-4.0 mug ml(-1) for both bovine serum albumin (BSA) and bovine hemoglobin (HEM), the detection limits were 10.4 ng ml(-1) (S/N = 3) for BSA and 11.4 ng ml(-1) (S/N = 3) for HEM. Samples were determined satisfactorily. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：869 / 874

页数：6

共 21 条

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BRADFORD MM, 1987, ANAL BIOCHEM, V163, P476

[2] CHROMOGENIC GROUPINGS IN THE LOWRY PROTEIN DETERMINATION [J].