An in vivo and in vitro structure-function analysis of the Saccharomyces cerevisiae U3A snoRNP: Protein-RNA contacts and base-pair interaction with the pre-ribosomal RNA

The structure and accessibility of the S. cerevisiae U3A snoRNA was studied In semi-purified U3A snoRNPs using both chemical and enzymatic probes and in vivo using DMS as the probe, The results obtained show that S. cerevisiae U3A snoRNA is composed of a short 5' domain with. two stem-loop structures containing the phylogenetically conserved boxes Al and A and a large cruciform 3' domain containing boxes B, C, C' and D. A precise identification of RNA-protein contacts is provided. Protection by proteins in the snoRNP and ii? vivo are nearly identical and were exclusively found in the 3' domain. There are two distinct protein anchoring sites: (i), box C' and its surrounding region, this site probably includes box D, (ii) the boxes B and C pail and the bases of stem-loop 2 and 4. Box C' is wrapped by the proteins, RNA-protein interactions are more loose at the level of boxes C and D and a box C and D interaction is preserved in the snoRNP. In accord with this location of the protein binding sites, an it? vivo mutational analysis showed that box Ct is important for U3A snoRNA accumulation, whereas mutations in the 5' domain have little effect on RNA stability. Our in viva probing experiments strongly suggest that, in exponentially growing cells, most of the U3A snoRNA molecules are involved in the 10-bp interaction with the 5'-ETS region and in two of the interactions recently proposed with 18 S rRNA sequences. Ow experimental study leads to a slightly revised version of the model of interaction proposed by J. Hughes. Single-stranded segments linking the heterologous helices are highly sensitive to DMS in vivo and their functional importance was tested by a mutational analysis. (C) 1997 Academic Press Limited.