Infrequent hMSH2 mutations in sporadic gastric adenocarcinoma with microsatellite instability

被引：31

作者：

Wu, MS

Sheu, JC

Shun, CT

Lee, WJ

Wang, JT

Wang, TH

Cheng, AL

Lin, JT

机构：

[1] NATL TAIWAN UNIV,COLL MED,DEPT INTERNAL MED,TAIPEI 10016,TAIWAN

[2] NATL TAIWAN UNIV,COLL MED,DEPT SURG,TAIPEI 10016,TAIWAN

来源：

CANCER LETTERS | 1997年 / 112卷 / 02期

关键词：

stomach; neoplasm; gastric adenocarcinoma; microsatellite instability; hMSH2; mutation;

D O I：

10.1016/S0304-3835(96)04565-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The status of genetic instability was determined with seven microsatellite markers from 40 patients with primary gastric adenocarcinoma. For those cases with microsatellite instability, alterations oi hMSH2 were further investigated by direct sequencing of reverse transcription-polymerase chain reaction products. Twelve (30%) of 40 patients were found to have microsatellite instability. Among them, one patient (1/6, 16.7%) was early gastric cancer and 11 (11/34, 32.4%) were advanced gastric cancer. There were seven patients with diffuse type (7/18, 38.7%), while five (5/22, 22.7%) were intestinal type tumors. The entire coding region of the hMSH2 gene in these 12 affected individuals was amplified and sequenced. Only a 41-year-old female patient with diffuse type advanced gastric cancer showed a GCT to TCT missense mutation al codon 207 with predicted protein change from alanine to serine. Our results indicate that genetic instability plays an important role in gastric tumorigenesis and alterations of the hMSH2 gene are related to only a small portion of sporadic gastric adenocarcinoma with microsatellite instability. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：161 / 166

页数：6

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