Biosynthesis of β-sitosterol and stigmasterol in Croton sublyratus proceeds via a mixed origin of isoprene units

被引:127
作者
De-Eknamkul, W [1 ]
Potduang, B [1 ]
机构
[1] Chulalongkorn Univ, Dept Pharmacognosy, Fac Pharmaceut Sci, Bangkok 10330, Thailand
关键词
Croton sublyratus Kurz; euphorbiaceae; callus culture; mevalonate pathway; deoxyxylulose pathway; beta-sitosterol; stigmasterol; mixed isoprene units;
D O I
10.1016/S0031-9422(02)00555-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A green callus culture of Croton sublyratus Kurz established from the leaf explants appeared to actively synthesize two well-known phytosterols, P-sitosterol and stigmasterol. The phytosterol biosynthesis was highly active during the linear phase of the culture. Feeding of [1-C-13]glucose into the callus culture at this growth phase showed that the label from glucose was highly incorporated into both phytosterols. Isolation of the labeled products followed by C-13 NMR analysis revealed that the phytosterols had their C-13-labeling patterns consistent with the acquisition of isoprene units via both the mevalonate pathway and the deoxyxylulose pathway with relatively equal contribution. Since the biosynthesis of phytosterol has so far been reported to be mainly from the classical mevalonate pathway, this study provides a new evidence on the biosynthesis of phytosterols via the novel deoxyxylulose pathway. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:389 / 398
页数:10
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