Survey of aflatoxin B-1 and ochratoxin A in commercial green coffee beans by high-performance liquid chromatography linked with immunoaffinity chromatography

被引：102

作者：

Nakajima, M ^{[1
]}

Tsubouchi, H ^{[1
]}

Miyabe, M ^{[1
]}

Ueno, Y ^{[1
]}

机构：

[1] SCI UNIV TOKYO, FAC PHARMACEUT SCI, DEPT TOXICOL & MICROBIAL CHEM, TOKYO 162, JAPAN

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 1997年 / 9卷 / 02期

关键词：

aflatoxin B-1; ochratoxin A; immunoaffinity chromatography; green coffee beans;

D O I：

10.1080/09540109709354938

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The natural occurrence of aflatoxin B-1 (AFB(1)) and ochratoxin A (OTA) has been surveyed in 47 samples of commercial green coffee beans. Samples were collected in Nagoya City, Japan, during 1988-93. Using a combination of immunoaffinity chromatography and high-performance liquid chromatography analysis, AFB(1) and OTA were quantified with detection limits of 2 ng kg(-1) and 0.1 mu g kg(-1) respectively. Positive rates and levels of AFB(1) and OTA were 32% and 2.0-32.9 ng kg(-1), and 30% and 0.1-17.4 mu g kg(-1) respectively. The samples contaminated with AFB(1) or OTA were mainly imported from African and Asian countries, and several samples were found to be positive for these two mycotoxins for the first time in Japan, although their levels were low.

引用

页码：77 / 83

页数：7

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