Detection and characterization of 20-oxosteroids in rat brains using LC-electron capture APCI-MS after derivatization with 2-nitro-4-trifluoromethylphenylhydrazine

被引：24

作者：

Higashi, T ^{[1
]}

Takido, N ^{[1
]}

Shimada, K ^{[1
]}

机构：

[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan

来源：

ANALYST | 2003年 / 128卷 / 02期

关键词：

MASS-SPECTROMETRY; NEUROSTEROIDS; DEHYDROEPIANDROSTERONE; PREGNENOLONE; STEROIDS;

D O I：

10.1039/b212375d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The detection and characterization of 20-oxosteroids in rat brains by liquid chromatography (LC)-electron capture atmospheric pressure chemical ionization (ECAPCI)-mass spectrometry (MS) is described. In the present method, oxosteroids were derivatized with a highly electron-affinitive reagent, 2-nitro-4-trifluoromethylphenylhydrazine, to convert them to the corresponding hydrazones. The derivatized steroids showed over 20-fold higher sensitivity in ECAPCI-MS than intact steroids measured in positive-APCI-MS. A brain sample was homogenized, purified with two disposable cartridges, derivatized and subjected to LC-ECAPCI-MS. Pregnenolone, progesterone, 5-dihydroprogesterone, allopregnanolone and epiallopregnanolone were clearly detected with a 50 mg sample of the brain tissue.

引用

页码：130 / 133

页数：4

共 13 条

[1]

Alomary AA, 2001, INT REV NEUROBIOL, V46, P97

[2]

Baulieu EE, 2001, INT REV NEUROBIOL, V46, P1

[3] LC-Electron capture APCI-MS for the determination of nitroaromatic compounds [J].