Screening for novel enzymes for biocatalytic processes: accessing the metagenome as a resource of novel functional sequence space

被引：151

作者：

Lorenz, P ^{[1
]}

Liebeton, K ^{[1
]}

Niehaus, F ^{[1
]}

Eck, J ^{[1
]}

机构：

[1] BRAIN Aktiengesell, D-64673 Zwingenberg, Germany

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2002年 / 13卷 / 06期

关键词：

D O I：

10.1016/S0958-1669(02)00345-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Historically, biotechnology has missed up to 99% of existing microbial resources by using traditional screening techniques. Strategies of directly cloning 'environmental DNA' comprising the genetic blueprints of entire microbial consortia (the so-called 'metagenome') provide molecular sequence space that along with ingenious in vitro evolution technologies will act synergistically to bring a maximum of available sequence-space into biocatalytic application.

引用

收藏

页码：572 / 577

页数：6

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