Redox potentials of the blue copper sites of bilirubin oxidases

被引：120

作者：

Christenson, Andreas

Shleev, Sergey ^{[1
]}

Mano, Nicolas

Heller, Adam

Gorton, Lo

机构：

[1] Univ Texas, Dept Chem Engn, Austin, TX 78712 USA

[2] Univ Texas, Texas Mat Inst, Austin, TX 78712 USA

[3] Lund Univ, Dept Analyt Chem, SE-22100 Lund, Sweden

[4] CNRS, Ctr Rech Paul Pascal, F-33600 Pessac, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2006年 / 1757卷 / 12期

关键词：

bilirubin oxidase; copper enzyme; redox potential; T1; T2; T3; sites; redox titration; spectroelectrochemistry;

D O I：

10.1016/j.bbabio.2006.08.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The redox potentials of the multicopper redox enzyme bilirubin oxidase (BOD) from two organisms were determined by mediated and direct spectroelectrochemistry. The potential of the T1 site of BOD from the fungus Myrothecium verrucaria was close to 670 mV, whereas that from Trachyderma tsunodae was > 650 mV vs. NHE. For the first time, direct electron transfer was observed between gold electrodes and BODs. The redox potentials of the T2 sites of both BODs were near 390 mV vs. NHE, consistent with previous finding for laccase and suggesting that the redox potentials of the T2 copper sites of most blue multicopper oxidases are similar, about 400 mV (c) 2006 Elsevier B.V. All rights reserved.

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页码：1634 / 1641

页数：8

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