Optical monitoring of oxygen tension in cortical microvessels with confocal microscopy

被引：40

作者：

Yaseen, Mohammad A. ^{[1
]}

Srinivasan, Vivek J. ^{[1
]}

Sakadzic, Sava ^{[1
]}

Wu, Weicheng ^{[1
]}

Ruvinskaya, Svetlana ^{[1
]}

Vinogradov, Sergei A. ^{[2
]}

Boas, David A. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp,Photon Migrat Imaging Lab, MGH MIT HMS Athinuola A Martinos Ctr Biomed Imagi, Charlestown, MA 02129 USA

[2] Univ Penn, Dept Biochem & Biophys, Philadelphia, PA 19104 USA

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 25期

关键词：

IN-VIVO; PHOSPHORESCENCE; TISSUE; VESSELS; CORTEX;

D O I：

10.1364/OE.17.022341

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Evaluating cerebral oxygenation is of critical importance for the understanding of brain function and several neuropathologies. Although several techniques exist for measuring cerebral oxygenation in vivo, the most widely accepted techniques offer limited spatial resolution. We have developed a confocal imaging system for minimally invasive measurement of oxygen tension (pO(2)) in cerebral microvessels with high spatial and temporal resolution. The system relies on the phosphorescence quenching method using exogenous porphyrin-based dendritic oxygen probes. Here we present high-resolution phosphorescence images of cortical microvasculature and temporal pO(2) profiles from multiple locations in response to varied fraction of inspired oxygen and functional activation. (C) 2009 Optical Society of America

引用

页码：22341 / 22350

页数：10

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